Microalgae and bacterium sorting device based on combination of deterministic lateral displacement and dielectrophoresis technology
A technology of lateral displacement and sorting device, which is applied in the direction of microorganism-based methods, enzymology/microbiology devices, specific-purpose bioreactors/fermenters, etc., which can solve the problems of low separation precision, low flux and easy clogging and other problems, to achieve the effect of improving separation throughput, simple operation and high degree of automation
Pending Publication Date: 2022-06-03
DALIAN JIAOTONG UNIVERSITY
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Problems solved by technology
[0005] In order to overcome the shortcomings and deficiencies of the existing single technology, the invention provides a high-throughput, high-precision multi-stage sorting device for microalgae cells and bacteria by using multiple technologies in combination. The problems of low throughput, low separation precision, easy clogging and high cost in cell separation technology will have important scientific significance and practical value for the field of microalgae separation and the application of ship ballast water detection
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Embodiment 1
[0035] like figure 1 As shown, a microalgae and bacteria sorting device based on deterministic lateral displacement and dielectrophoresis technology includes a PDMS cover layer 1, an ITO electrode layer 2 and a glass substrate layer 3; the ITO electrode layer 2 is deposited on the glass substrate On the bottom layer 3, the PDMS cover layer 1 is closely bonded to the glass substrate layer 3;
[0036] The PDMS cover sheet layer 1 adopts soft photolithography technology to make the base layer, which is divided into several steps of cleaning, pre-baking, spin coating, middle baking, exposure, post-baking, and development, and then, on the base layer, use PDMS to cast made.
[0037] The PDMS cover sheet layer 1 includes a sequentially connected DLD sorting module 1-1, a buffer anti-clogging module 1-2 and a DEP array sorting module 1-3;
[0038] like figure 2As shown, the DLD sorting module of the present invention comprises a DLD sorting channel, a sample inlet 1-1-1, a sheat...
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The invention discloses a microalgae and bacterium sorting device based on deterministic lateral displacement and a dielectrophoresis technology, and belongs to the technical field of cell filtering and sorting. The device comprises a PDMS cover plate layer, an ITO electrode layer and a glass substrate layer. The PDMS cover sheet layer comprises a deterministic lateral displacement sorting module, a buffer anti-blocking module and a dielectrophoresis array sorting module, the deterministic lateral displacement sorting module can realize preliminary separation of microalgae cells and bacteria according to the size, and the buffer anti-blocking module prevents cell agglomeration and countercurrent through a designed arrow-shaped microcolumn array; according to the dielectrophoresis array sorting module, high-flux sorting is achieved through a designed parallel eight-channel dielectrophoresis sorting array, the problem that flow speeds needed by deterministic lateral displacement separation and dielectrophoresis separation are not matched is solved, and strong and stable dielectrophoresis force is provided for cells through designed comb-shaped electrodes and wheat-ear-shaped electrodes; therefore, high-precision and high-efficiency separation of microalgae cells and residual bacteria is realized.
Description
technical field [0001] The invention belongs to the technical field of cell filtration and sorting, and in particular relates to a multistage sorting device for microalgae cells and bacteria based on the combination of deterministic lateral displacement and dielectrophoresis technology. Background technique [0002] Microfluidic chip technology integrates basic operations such as sample preparation, reaction, sorting, and detection into microscale channels, and automatically completes the analysis and processing process. It has the advantages of less sample amount, small size, low cost, high efficiency, easy to carry, easy to integrate, etc. It has been widely used in various research fields such as biology, medicine, chemistry, agriculture, food, environment, and navigation. [0003] Microfluidic particle separation technology is mainly divided into two categories: active separation and passive separation. Common technologies for active separation methods include dielectro...
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Login to View More IPC IPC(8): C12M1/42C12M1/00C12N1/02B01L3/00C12R1/19C12R1/89
CPCC12M23/16C12M47/04C12M35/02C12N1/02B01L3/5027B01L2200/12B01L2400/0424
Inventor 王艳娟程昊文
Owner DALIAN JIAOTONG UNIVERSITY