Preparation method of monodisperse carboxyl colloidal carbon and application of monodisperse carboxyl colloidal carbon in novel coronavirus antibody detection test paper
A coronavirus and colloidal carbon technology, applied in the biological field, can solve problems such as inability to act, and achieve the effects of low cost, stable product particle size, and easy availability of raw materials
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Embodiment 1
[0050] The monodisperse carboxyl colloidal carbon of the present embodiment is prepared by the following preparation method:
[0051] S1, ultrasonic treatment: after taking 1g of carbon black and 30mL of ultrapure water, set 800W of power ultrasonic treatment for 30min to obtain 30mL of carbon black suspension;
[0052] S2, oxidation reaction: dilute the carbon black suspension obtained in step S1 by 1 times, and then slowly add 2.5mL of 69.8% concentrated nitric acid to the diluted carbon black suspension and stir evenly; Ultrapure water was added to the volume to 60mL, and then the reaction system was transferred to a 100mL single-mouth flask, connected to the condensing reflux tube, and heated and condensed reflux in an oil bath at 105 °C for 5h;
[0053] S3, centrifugation: the solution obtained from step S2 is centrifuged five times;
[0054] The parameters of the first centrifugation are 3000 rpm centrifugation for 5 min; The parameters of the second centrifugation are 6000 ...
Embodiment 2
[0059]The present embodiment is substantially the same as Example 1, the only difference is: the oxidation reaction of step S2 is: the carbon black suspension obtained in step S1 is heated to boiling, and then 15mL of 0.05M potassium permanganate solution is slowly added dropwise to the diluted carbon black suspension and stirred evenly, and then the reaction system is transferred to a 100mL single-mouth flask, connected to a condensing return pipe, heated and condensed back into the 1h oil bath at 105 ° C;
Embodiment 3
[0060] Example 3: Prepare a new coronavirus anti-physical examination test paper
[0061] Novel coronavirus anti-physical examination test paper comprises PVC plate 1, the surface of the PVC plate 1 is successively pasted sample pad 2, binding pad 3, filtration membrane 4, nitrocellulose membrane 8, absorption pad 9, the nitrocellulose membrane 8 is provided with a detection line G line 6, a detection line M line 5 and a quality control line C line 7, the binding pad 3 is coated with colloidal carbon - novel coronavirus recombinant antigen complex and colloidal carbon - chicken IgY antibody complex, the detection line G line 6 is covered with a rat anti-human IgG secondary antibody, The detection line M line 5 is coated with a rat anti-human IgM secondary antibody, the quality control line is coated with rabbit anti-chicken IgY secondary antibody;
[0062] The colloidal carbon - novel coronavirus recombinant antigen complex and colloidal carbon - chicken IgY antibody complex are p...
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