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Eucommia ulmoides seed meal polypeptide and application thereof in anti-fatigue product/preparation of anti-fatigue product

An anti-fatigue and seed meal technology is applied in the application field of Eucommia ulmoides seed meal polypeptide in anti-fatigue products/preparation of anti-fatigue products, and achieves significant anti-fatigue effect, excellent anti-fatigue effect, and improved economic benefits.

Active Publication Date: 2022-06-07
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Regarding whether the polypeptide of eucommia seed meal has the effect of anti-fatigue, the above-mentioned patent literature does not mention

Method used

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  • Eucommia ulmoides seed meal polypeptide and application thereof in anti-fatigue product/preparation of anti-fatigue product
  • Eucommia ulmoides seed meal polypeptide and application thereof in anti-fatigue product/preparation of anti-fatigue product
  • Eucommia ulmoides seed meal polypeptide and application thereof in anti-fatigue product/preparation of anti-fatigue product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of eucommia seed meal polypeptide having an anti-fatigue effect comprises the following steps:

[0039](1) Take eucommia seed meal crushed, sieved, add 20 weight times (V / m) of 70% ethanol soaked to extract 36h, filtered, to obtain filtrate A and precipitation A, the precipitation A at 60 °C low temperature drying, standby;

[0040] (2) Add precipitate A to 25 wt times (V / m) of water, extract 3h in a water bath at 60 °C, filter, and obtain filtrate B and precipitate B;

[0041] (3) Add 25 weight times (V / m) of water to precipitate B, adjust the pH value to 9 with 0.5mol / L sodium hydroxide solution, stir and extract at a constant temperature at 50 °C for 4h, and then centrifuge at 3500 rpm / min for 15 min to obtain supernatant A and precipitate C, adjust the supernatant A pH value to 4 with 0.5mol / L hydrochloric acid solution, and then centrifuge at a speed of 3500 rpm / min for 20 min to obtain supernatant B and precipitate D. After resolving the precipi...

Embodiment 2

[0044] (1) Take eucommia seed meal crushed, sieved, add 10 weight times (V / m) of 90% ethanol soaked to extract 24h, filtered, to obtain filtrate A and precipitation A, the precipitation A at 50 °C low temperature drying, standby;

[0045] (2) Add sediment A to 15 weight times (V / m) of water, extract 2h in a water bath at 80 °C, filter, and obtain filtrate B and sediment B;

[0046](3) Add 15 weight times (V / m) of water to precipitate B, adjust the pH value to 9 with 0.1mol / L sodium hydroxide solution, stir at a constant temperature at 40 °C to extract 3h, and then centrifuge at 2500 rpm / min for 10 min to obtain supernatant A and precipitate C, adjust the supernatant A pH value with 0.1mol / L hydrochloric acid solution to 5, and then centrifuge for 10 min at a speed of 2500 rpm / min to obtain supernatant B and precipitate D. After resolving precipitate D with water, the pH was adjusted to 6.5, and after pre-freezing at -40 °C for 20 min, the vacuum was freeze-dried for 48 h to obtain...

Embodiment 3

[0049] (1) Take eucommia seed meal crushed, sieved, add 30 weight times (V / m) of 80% ethanol soaked to extract 24h, filtered, to obtain filtrate A and precipitation A, the precipitation A at 70 °C low temperature drying, standby;

[0050] (2) Add precipitate A to 20 times by weight (V / m) of water, extract 4h in a water bath at 70 °C, filter, and obtain filtrate B and precipitate B;

[0051] (3) Add 20 weight times (V / m) of water to precipitate B, adjust the pH value to 10 with 1mol / L sodium hydroxide solution, stir at a constant temperature at 60 °C to extract for 6h, and then centrifuge at 5000 rpm / min for 20 min to obtain supernatant A and precipitate C, adjust the supernatant A pH value with 1mol / L hydrochloric acid solution to 4, and then centrifuge at a speed of 5000 rpm / min for 20 min to obtain supernatant B and precipitate D. After resolving precipitate D with water, adjust the pH to 7, pre-freeze at -40 °C for 20 min, freeze-dry in vacuo for 48 h to obtain Eucommia seed me...

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Abstract

The invention discloses eucommia seed meal polypeptide and application thereof in an anti-fatigue product / preparation of the anti-fatigue product. The eucommia seed meal polypeptide is prepared by the following method: taking eucommia seed meal, crushing, sieving, soaking with ethanol, extracting, and filtering to obtain a precipitate A; extracting the precipitate A in a water bath and filtering to obtain a precipitate B; adding water to redissolve the precipitate B, adjusting the pH value, centrifuging to obtain a precipitate C and a supernatant A, adjusting the pH value of the supernatant A, centrifuging to obtain a precipitate D, adding water to redissolve the precipitate D, adjusting the pH value, and drying to obtain eucommia seed meal protein; heating, adjusting the pH value, performing enzymolysis, performing enzyme deactivation and performing centrifugation to obtain supernate C; adjusting the pH value of the supernate C, centrifuging to obtain supernate D, and drying the obtained eucommia seed meal polypeptide solution to obtain the eucommia seed meal polypeptide. The anti-fatigue effect of the eucommia seed meal polypeptide is verified through indexes such as mouse load swimming experiments, blood lactic acid, serum urea nitrogen and muscle glycogen, and the result shows that the eucommia seed meal polypeptide has a remarkable anti-fatigue effect.

Description

Technical field [0001] The present invention belongs to the field of new uses of polypeptides, specifically relates to the application of eucommia seed meal polypeptides in anti-fatigue products / preparation of anti-fatigue products. Background [0002] Regarding the polypeptide of eucommia seed meal with anti-fatigue effect, the following literature has been disclosed: [0003] CN 112402480A discloses a method and application of eucommia male flower extract having anti-fatigue effect, the steps thereof are: take a certain amount of eucommia male flowers, eucommia male flowers with 15 times the mass of water extraction, 100 ° C reflux for 2 hours, filter to take the filtrate for use, and then add 15 times the mass water to the eucommia male flowers, boiling and reflux secondary, filtering to collect the filtrate, mixing the filtrate twice, concentrating spray drying, to obtain eucommia male flower extract. The present invention is extracted from water, filtered and concentrated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C12P21/06C07K1/30C07K1/34A23C9/13A23G3/36A23G3/44A23L2/39A23L2/52A23L33/18A61K38/01A61P39/00
CPCC07K2/00C12P21/06C07K1/34C07K1/30A61K38/011A61P39/00A23L33/18A23L2/39A23L2/52A23C9/1322A23G3/44A23G3/364A23V2002/00A23V2200/30A23V2250/55Y02P60/87
Inventor 李斌高丽颖宋燃王敏竹王彤黄山
Owner QINGDAO UNIV OF SCI & TECH
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