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Tissue culture and rapid propagation seedling raising method of pyrola rotundifolia

A technology for rapid breeding of seedlings and staghorn grass, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low reproduction coefficient, long reproduction cycle, and divide into 2~3 plants, so as to ensure stable genetics. , the effect of stable genetic traits

Active Publication Date: 2022-07-01
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, Yunnan, Hebei and other provinces and cities have carried out the introduction and domestication of wintergreen wild resources, but due to the dormancy of its seeds, it cannot germinate under the current technical conditions, and only 2~3 plants can be divided into 1 plant by division. The reproductive cycle is at least 1 year, and there are disadvantages such as low reproductive coefficient and long reproductive cycle.
Master thesis published by Zhao Yao ("Research on the Introduction of Wintergreen" [D]. Baoding City: Hebei Agricultural University, 2005: 10~13) and published by Wang Wei, Lu Jiantao, etc. ("Research on Tissue Culture of Wintergreen" [J ], Hebei Agricultural Science and Technology, 2008: 48) both proposed tissue culture research on wintergreen, but they were all limited to research on disinfection technology, and did not conduct a series of research and reports on primary induction, subculture proliferation and rooting culture

Method used

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  • Tissue culture and rapid propagation seedling raising method of pyrola rotundifolia
  • Tissue culture and rapid propagation seedling raising method of pyrola rotundifolia
  • Tissue culture and rapid propagation seedling raising method of pyrola rotundifolia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Selection of explants: Transplant staghorn grass indoors, spray the stems and leaves with 75% carbendazim 1000 times liquid after turning green, spray once every 7 days, spray 3 times continuously, and then dig out The whole plant, the roots and leaves below the soil surface were removed, and the stem segments with axillary buds and the axillary buds at the base were used as explants.

[0027] 2. Cleaning of explants: Dip saturated soapy water solution with a brush, repeatedly brush the surface until there is no residue of soil, then add an appropriate amount of water to the beaker and add 3 drops of Tween-80 dropwise, shake for 10 minutes, and rinse with tap water for 60 minutes .

[0028] 3. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% alcohol for 10s, rinse with sterile water for 3 times, disinfect with saturated bleach solution for 10nin, rinse with sterile water for 3 times , 8% hydrogen peroxide solutio...

Embodiment 2

[0033] 1. Selection of explants: Transplant staghorn grass indoors, spray the stems and leaves with 75% carbendazim 1000 times liquid after turning green, spray once every 7 days, spray 3 times continuously, and then dig out The whole plant, the roots and leaves below the soil surface were removed, and the stem segments with axillary buds and the axillary buds at the base were used as explants.

[0034] 2. Cleaning of explants: Dip saturated soapy water solution with a brush, repeatedly brush the surface until there is no residue of soil, then add an appropriate amount of water to the beaker and add 3 drops of Tween-80 dropwise, shake for 13 minutes, and rinse with tap water for 65 minutes .

[0035] 3. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% alcohol for 13s, rinse with sterile water for 3 times, disinfect with saturated bleach solution for 13nin, rinse with sterile water for 4 times , 8% hydrogen peroxide solutio...

Embodiment 3

[0040] 1. Selection of explants: Transplant staghorn grass indoors, spray the stems and leaves with 75% carbendazim 1000 times liquid after turning green, spray once every 7 days, spray 3 times continuously, and then dig out The whole plant, the roots and leaves below the soil surface were removed, and the stem segments with axillary buds and the axillary buds at the base were used as explants.

[0041] 2. Cleaning of explants: Dip the saturated soapy water solution with a brush, repeatedly brush the surface until there is no residue of soil, then add an appropriate amount of water to the beaker and add 3 drops of Tween-80 dropwise, shake for 15 minutes, and rinse with tap water for 70 minutes. .

[0042] 3. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% alcohol for 15s, rinse with sterile water for 3 times, disinfect with saturated bleach solution for 15nin, rinse with sterile water for 4 times , 8% hydrogen peroxide so...

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Abstract

The invention belongs to the field of traditional Chinese medicinal materials and tissue culture, and particularly discloses a pyrola rotundifolia tissue culture seedling raising method which comprises the following steps: selecting pyrola rotundifolia axillary buds as explants, sterilizing, inoculating to a primary cluster bud induction culture medium to induce cluster buds, and then transferring to a subculture multiplication culture medium to obtain more cluster buds. According to the method, the wintergreen seedlings are produced by utilizing a tissue culture technology, the method belongs to a modern biotechnology, is a factory seedling culture method carried out in an artificial controllable environment, is not limited by weather and seasons, and can provide a large number of related seedlings with stable quality in a short time.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicinal materials and tissue culture, and particularly relates to a method for cultivating seedlings in groups of S. chinensis. Background technique [0002] Pyrola calliantha, also known as Meihua Pyrola, Luhancao, Luohan tea, evergreen tea, etc., grows in mountain coniferous forests, coniferous and broad-leaved mixed forests or broad-leaved forests at an altitude of 700-4100 meters. Mainly distributed in Yunnan, Sichuan, Guizhou, Tibet, Shaanxi, Qinghai, Gansu, Shanxi, Shandong, Hebei, Henan, Anhui, Jiangsu, Zhejiang, Fujian, Hubei, Hunan and Jiangxi. Using the whole herb as medicine, it has the functions of dispelling rheumatism, strengthening muscles and bones, hemostasis, and relieving cough. [0003] Deerwort contains dozens of active ingredients, such as crystal blue glycosides, cetosides, cetosides, high arbutin, hypericin, quercetin, hydroxykidney cetosides, gallic acid, etc. Among ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02A50/30
Inventor 许宗亮张金渝曾祥飞杨天梅朱新焰赵露琴王丽冯人和李邵兴
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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