Serum metabolism marker related to pulmonary tuberculosis and application thereof

Abstract

The invention discloses a serum metabolism marker related to pulmonary tuberculosis and application of the serum metabolism marker related to the pulmonary tuberculosis, and the serum metabolism marker related to the pulmonary tuberculosis comprises at least one of 3-oxopalmitic acid, akebia stem saponin, thiolithocholic acid, 2-decyl furan, (decahydro-4, 8, 8-trimethyl-1, 4-methylene azulene-9-subunit) methanol, D-(+)-camphor and 2-methylamino adenosine, and the serum metabolism marker related to the pulmonary tuberculosis comprises at least one of 3-oxopalmitic acid, akebia stem saponin, thiolithocholic acid, 2-decyl furan, (decahydro-4, 8, 8-trimethyl-1, 4-methylene azulene-9-subunit) methanol, D-(+)-camphor and 2-methylamino adenosine. According to the application, the serum metabolism marker is used for preparing a detection reagent or applied to detection related to pulmonary tuberculosis. The serum metabolism marker is suitable for detection related to pulmonary tuberculosis, the accuracy of the detection result is high, and the detection efficiency can be improved.

Description

technical field [0001] The invention relates to a serum metabolic marker related to pulmonary tuberculosis and an application thereof, belonging to the technical field of biological detection. Background technique [0002] Tuberculosis (TB) is a widespread and in many cases fatal chronic infectious disease caused by Mycobacterium tuberculosis (Mtb) infection. most. A quarter of the global population is infected with Mycobacterium tuberculosis but has no clinical manifestations of TB, and 5-15% of them may develop active pulmonary TB. [0003] Early diagnosis of tuberculosis and timely anti-tuberculosis treatment are of great significance to effectively control the progression of tuberculosis and the spread of Mycobacterium tuberculosis. There are currently limited methods for diagnosing TB infection, mainly including sputum smear, sputum bacterial liquid culture, Mycobacterium tuberculosis skin test (TST), radioactive X-rays, serological antibody antigen immunoassays, and ...

AI Technical Summary

Problems solved by technology

For example, although microscopic examination of sputum smear staining is simple and easy, in most cases, Mycobacterium tuberculosis may not be found in the sputum of tuberculosis patients, so the detection rate of microscopic examination is low

In addition, the liquid culture method can slightly increase the positive rate, but it takes too long (2-6 weeks) and requires a specialized biosafety level 3 laboratory

In terms of immunological diagnosis, the Mycobacterium tuberculosis pure protein derivative (PPD) used in the Mycobacterium tuberculosis skin test (TST) contains many mycobacterial species (including pathogenic mycobacteria, environmental mycobacteria Therefore, the specificity of PPD diagnosis of tuberculosis is poor, and it is impossible to accurately distinguish whether a positive PPD test result is due to BCG vaccination, sensitization after exposure to a variety of non-tuberculous mycobacteria in the environment, or true tuberculosis mycobacteria caused by bacillus infection

Other immunological diagnostic techniques, such as T-SPOT.TB kit and QuantiFERON-TB Gold kit, although the accuracy has been improved, but the operation is complicated, expensive, and the cost is extremely high, which seriously restricts its application to millions of patients. Large-scale tuberculosis screening and diagnosis for millions of active tuberculosis patients and hundreds of millions of latent tuberculosis infected people

In terms of PCR, the latest xpertMTB / RIF technology based on real-time PCR is significantly more sensitive than smear microscopy, but its high cost and high false positives in areas with low prevalence of rifampicin resistance limit its widespread use. application

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