Sorting method of fluorescence coding microspheres

Abstract

The invention discloses a sorting method of fluorescent coded microspheres, which comprises the following steps: mixing different coded microspheres in a to-be-detected sample, and respectively grabbing corresponding different target molecules to obtain to-be-detected liquid; controlling the to-be-detected liquid to sequentially enter an accumulation area, a real-time detection area, a light pattern conversion area and a sorting separation area of the photoelectric chip through a fluid control system, so as to realize sorting of the fluorescent coded microspheres and corresponding to-be-detected molecules. According to the invention, the direction of dielectrophoretic force is changed by using the light guide material in combination with the light pattern design and changing the electric field distribution on the plane, so that the stress of the coding microspheres is changed, the purposes of positioning, grabbing, releasing and moving the coding microspheres are achieved, and the sorting and immunodetection efficiency of the coding microspheres is improved.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a sorting method for fluorescently encoded microspheres. Background technique [0002] The detection of many cancers is closely related to the concentration of cytokines, and sometimes a single cancer cell can cause the release of multiple cytokines. In clinical practice, the amount and concentration of interleukins detected in the serum of patients can be used as indicators for early cancer diagnosis and after treatment. For example, the measurement of cytokines such as IL-1, IL-2, IL-6, IL-8, IL-10, IL-15, and IL-17 can be used for clinical detection of liver cancer, lung cancer, pancreatic cancer and ovarian cancer. For lung, breast, and ovarian cancers, most treatment times are less than 1 year from disease detection to ineffective treatment. If multiple cytokines can be detected in serum, the accuracy of cancer diagnosis and the survival rate of cancer patients can be i...

AI Technical Summary

Problems solved by technology

[0006] The general metal dielectrophoretic force design, using micro-electromechanical process, first deposits a metal layer on the glass surface, then defines the shape of the electrode by photoresist, after etching and developing, it can form a metal pattern (conductive layer) at the micron scale, and then in The electrophoretic force is generated in the channel (microfluidic pipeline), but the pattern of the metal electrode is fixed, and the shape of the electric field is also fixed, so the flexibility is not enough, and the manufacturing process is also complicated, requiring special manufacturing technology and training

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