Tissue bank specimen cryopreservation liquid and application thereof

Abstract

The invention relates to a tissue bank specimen cryopreservation solution and application thereof, and belongs to the technical field of biomedicine. By mixing different salt solutions, a normal cell microenvironment of a human body is simulated, extracellular fluid is simulated, normal cell osmotic pressure is maintained, tissue cell edema or dehydration is avoided, and the most basic living space is provided for biomacromolecules; by adding guanidinium thiocyanate and ammonium thiocyanate, the activity of RNAse (RNA hydrolase) is reduced, the survival state of an RNA chain is improved, and RNA which is easier to degrade compared with protein and DNA is protected; by adding the glycerin component, tissue surface tension is maintained, specimen glossiness is maintained, decay is prevented, the refractive index is increased, and the fluidity of the preserving fluid is guaranteed.

Description

technical field [0001] The invention relates to a tissue bank specimen cryopreservation solution and its application, belonging to the technical field of biomedicine. Background technique [0002] In hospitals, medical schools, and scientific research units, human tissue banks, including organs and various human tissue cell banks, are often established for medical treatment, teaching and scientific research tasks. The tissue bank takes the sample as the center, and integrates macroscopic data such as patient information and follow-up data with microscopic data such as DNA, RNA and protein, and provides high-level sample support for future scientific research. At present, nearly 80% of biological samples in hospitals are preserved and used by corresponding departments and pathology departments. The main storage containers are -80℃ ultra-low temperature refrigerators and -196℃ liquid nitrogen tanks, and tap water is used as the preservation solution for specimens. The process...

AI Technical Summary

Problems solved by technology

However, in actual operation, repeated freezing and thawing of samples in the frozen state and repeated cooling and rewarming of the vitrified state will cause damage to the macromolecules and cells to be analyzed. The repeated freezing and thawing process will lead to a decrease in thermodynamic free energy and protein ice crystals. interaction, which accelerates the degradation of biological specimens; the cold chain used in the refrigerated transportation of biological specimens is expensive and prone to failure, which requires certain risks

In this way, the number of biological downstream molecules and markers at all levels will be inaccurate compared with the living body, and there will be a large bias in simulating the human body, and scientific research will cause a certain degree of unreliability

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