Preparation method of gamma-polyglutamic acid/chitosan microsphere scaffold

Abstract

The invention discloses a gamma-polyglutamic acid / chitosan microsphere scaffold preparation method, which comprises: S1, mixing gamma-polyglutamic acid and a NaOH solution, stirring, and completely dissolving to prepare a gamma-polyglutamic acid solution; s2, adding chitosan into the gamma-polyglutamic acid solution, and stirring to prepare a chitosan-gamma-polyglutamic acid mixed solution; s3, dropwise adding an acetic acid solution into the chitosan-gamma-polyglutamic acid mixed solution, and stirring until the solution is stable to obtain supernate and solid substances precipitated at the bottom; s4, pouring out the supernate, and flushing the solid substance with flushing fluid with the same pH value as the supernate; s5, the washed solid matter is sieved, and in the sieving process, it is always kept that the solid matter is immersed in supernate; s6, after sieving, standing, pouring out supernate, pouring into a centrifugal tube, standing again, completely sucking water by using a pipette, and freezing in a refrigerator at the temperature of 80 DEG C below zero; and S7, drying the frozen centrifugal tube containing the solid substance in a drying machine to obtain the gamma-polyglutamic acid-chitosan scaffold.

Description

technical field [0001] The present application relates to the technical field of microsphere scaffolds, in particular to a preparation method of a γ-polyglutamic acid / chitosan microsphere scaffold. Background technique [0002] Bone defect is a disease with a high incidence in orthopedic diseases, and the treatment of bone defect has always been a clinical problem. At present, autologous or allogeneic bone transplantation is still considered to be the most effective method to repair bone defects, but autologous bone transplantation has problems such as limited material availability, long operation time, high risk and high cost. risk of transmission of the disease to the recipient. Other commonly used bone repair techniques include distraction osteogenesis, bone cement filling, etc., but they are not perfectly applicable to the repair of various types of bone defects. Although the above protocols have been shown to improve bone defect repair, none of them have all the desir...

AI Technical Summary

Problems solved by technology

However, the emulsification crosslinking method needs to use emulsifiers (such as toluene, liquid paraffin, sherwood oil, etc.) and crosslinking agents (such as glutaraldehyde, formaldehyde, etc.), and the required reagents are not environmentally safe enough, and it is difficult for the solvent to be removed from the microsphere product at last. removal, there is a risk of human toxicity and environmental pollution, and the steps of the emulsification cross-linking method are relatively complicated, the reaction conditions are relatively harsh, and there are many factors affecting the preparation process of the microsphere scaffold. Difficult to control pore size etc.

[0007] The above defects make it difficult to produce and sell microspheres for bone tissue engineering on a large scale. Finding better bone tissue engineering materials and preparing safe and effective bone tissue engineering microspheres in an efficient and environmentally friendly manner have become urgent problems to be solved in the medical field.

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