Screening method for heterotrophic nitrification and aerobic denitrification bacteria in acid rice field soil
A technology of aerobic denitrification and heterotrophic nitrification, which is applied in the field of screening of heterotrophic nitrification and aerobic denitrification bacteria in acid paddy soil, can solve the problems of less isolation of heterotrophic nitrification and aerobic denitrification strains and low efficiency of strain screening, Achieve the effect of efficient removal of ammonia nitrogen and nitrate nitrogen, high removal rate and simplified screening steps
Active Publication Date: 2022-07-22
ZHEJIANG UNIV
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Problems solved by technology
There are many screening media for heterotrophic nitrifying and aerobic denitrifying bacteria reported so far, but there are few screening media for isolating heterotrophic nitrifying and aerobic denitrifying bacteria strains from acidic rice soil, and there are defects such as low efficiency of screening strains
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Embodiment 1
[0074] Embodiment 1, a kind of rice soil heterotrophic nitrification aerobic denitrification strain screening method, and its preparation method is to carry out the following steps in sequence:
[0075] 1), paddy soil heterotrophic nitrification liquid medium and denitrification liquid medium formula preparation:
[0076] In a 1L container, add the components in Table 1, and add sterile water to make up to 1000mL.
[0077] Table 1. Heterotrophic nitrification liquid medium formula
[0078]
[0079] Table 2. Recipe of Denitrification Solid Medium
[0080]
[0081]
[0082] Table 3. Formula of Nutrient Element Solution
[0083]
[0084] 2), Acidic paddy soil collection:
[0085] Take the acid rice field soil in Chengbei Fan, Shanghu Village, Yuhang Street, Yuhang District, Hangzhou City, Zhejiang Province as a sample, adopt a five-point sampling method, mix the samples evenly, and perform the following step 3).
[0086] 3) Screening of heterotrophic nitrifying st...
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The invention discloses a culture medium used in a screening method of heterotrophic nitrification and aerobic denitrification bacteria in acidic rice field soil. The culture medium comprises a heterotrophic nitrification liquid culture medium and a denitrification solid culture medium. The invention also discloses a method for screening the heterotrophic nitrification and aerobic denitrification bacteria in the acid rice field soil by using the culture medium. The method comprises the following steps: preparing a selective culture medium for the heterotrophic nitrification and aerobic denitrification bacteria in the acid rice field soil; preparing a rice field soil turbid liquid; screening of heterotrophic nitrification strains; separating a heterotrophic nitrification strain; purifying the heterotrophic nitrification strain; screening of heterotrophic nitrification and aerobic denitrification strains; detecting the heterotrophic nitrification and aerobic denitrification capabilities; and carrying out DNA extraction, PCR analysis and identification on the strain. The method can be used for screening heterotrophic nitrification and aerobic denitrification bacteria in acid rice field soil, and has the potential of efficiently removing ammonia nitrogen and nitrate nitrogen in a water body.
Description
technical field [0001] The invention relates to a screening method for heterotrophic nitrifying aerobic denitrifying bacteria in acidic paddy soil and a culture medium used therefor. Background technique [0002] Nitrogen is one of the main factors leading to water pollution and eutrophication in my country. The nitrogen concentration in the water exceeds the self-purification capacity of the water and reaches the level of destroying the original use, resulting in water pollution. Studies have shown that one of the main causes of disease in aquatic animals is that the content of nitrite nitrogen in aquaculture water is seriously exceeding the standard. Nitrous nitrogen can oxidize ferrohemoglobin in fish and shrimp, making it methemoglobin, causing it to lose its ability to transport oxygen, and finally cause fish and shrimp to suffocate to death. Therefore, nitrogen removal becomes the key to wastewater treatment. The physical and chemical methods are cumbersome and costl...
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IPC IPC(8): C12N1/02
CPCC12N1/02Y02W10/10
Inventor 张奇春刘小婷吴丹王京文
Owner ZHEJIANG UNIV