Automatic cell sorting system and method based on micro-droplet control technology

Abstract

The invention discloses an automatic cell sorting system based on a micro-droplet control technology and a method thereof. According to the invention, a microfluidic technology and an optical tweezers technology are combined, independent movement control is carried out on target cells in discrete tiny liquid drops, and the device has the advantages of high accurate controllability, low consumption of samples and reagents, short reaction time, pollution isolation and low skill requirements of operators; based on a programmable micro-droplet control technology, a cell recognition module for precise recognition and a cell control module capable of controlling cells in a suspension, full-automatic cell sorting is realized, manpower resources are saved, and hidden dangers of secondary infection are avoided; the micro-droplet control chip has the characteristic of high universality, is suitable for sorting target cells in a plurality of types of turbid liquid on the same micro-droplet control chip, and also can sort a plurality of target cells in the same turbid liquid at the same time; the invention provides a novel operation and analysis tool for life science research.

Description

technical field [0001] The present invention relates, in particular, to an automated cell sorting system based on microdroplet manipulation technology and a sorting method thereof. Background technique [0002] Digital microfluidics (DMF) technology is a new droplet manipulation technology that independently manipulates discrete droplets. The size of the manipulated droplets is generally in the microliter to picoliter level. Common driving methods include dielectric Wetting, dielectrophoresis, surface acoustic wave, electrostatic force, magnetic force, etc. Among them, the electronically manipulated digital microfluidic technology based on dielectric wetting can achieve precise control of discrete droplets by changing the contact angle of droplets on the hydrophobic surface, and can realize the integration of droplet distribution, movement, merging and splitting operations. Automation and automation are the most rapidly developing fluid-driven forms. DMF technology quickly...

AI Technical Summary

Problems solved by technology

However, the above method can only be applied to the sorting of cells with obvious differences in cell size and shape and capable of producing fluorescence or chemiluminescence, and the adaptability of cell types still has great limitations.

In addition, the above methods usually require professional laboratory equipment and personnel, a large amount of reagents and long operation time, and multi-step cumbersome operations and pre-treatments, which not only set a high threshold for non-professionals, but also cell sorting Results are heavily dependent on operator skills and experience

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