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Drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating as well as preparation method and application of drug-loaded ZIF-8 nanoparticles

A technology of TF-PEG-PLGA and COOH-PEG-PLGA, which is applied in the field of biomedical detection, can solve problems such as restrictive application and difficulty in complete surgical resection, so as to enhance permeability, improve drug water solubility, and reduce normal tissue drug distribution effect

Active Publication Date: 2022-08-05
十堰市太和医院(湖北医药学院附属医院)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the invasive growth pattern of GBM, it is difficult to completely remove it by surgery, and radiotherapy has the problem of radioresistance
The application of chemotherapy drugs can improve the prognosis of patients with surgery and radiotherapy, but the blood-brain barrier penetration ability of chemotherapy drugs and the side effects of chemotherapy restrict their application in GBM treatment

Method used

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  • Drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating as well as preparation method and application of drug-loaded ZIF-8 nanoparticles
  • Drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating as well as preparation method and application of drug-loaded ZIF-8 nanoparticles
  • Drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating as well as preparation method and application of drug-loaded ZIF-8 nanoparticles

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Experimental program
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Effect test

Embodiment approach

[0046] According to a typical embodiment of the present invention, there is provided a preparation method of drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating, the method comprising:

[0047] Step S101 , adding the target drug, 2-methylimidazole, and zinc nitrate into a methanol solution to mix for a first reaction, and then purifying and dissolving in methanol to obtain a drug-loaded ZIF-8 nanoparticle solution;

[0048] In the above technical scheme,

[0049] Described methanol solution is pure methanol solution;

[0050] The target drug includes aloe-emodin. In other embodiments, the target drug can also be cisplatin, paclitaxel, indocyanine green;

[0051] The mass ratio of the target drug, 2-methylimidazole and zinc nitrate is 1:(15-17):(7-8). This mass ratio range is beneficial to obtain the best drug encapsulation efficiency.

[0052]The conditions of the first reaction all include: magnetic stirring at a rotational speed of 200-300 rpm, reaction at r...

Embodiment 1

[0072] Embodiment 1. Drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating and preparation method thereof

[0073] Dissolve 75mg zinc nitrate in a 2.5ml sample bottle (bottle A), dissolve in a 120W ultrasonic bath for 2 minutes; take 165mg dimethylimidazole and 10mg aloe-emodin and dissolve them in 5ml methanol (bottle B), take a 120W ultrasonic bath for 5 minutes Dissolve and stir magnetically for 5 minutes at room temperature, 250 rpm. Add bottle A to bottle B dropwise, and at room temperature, keep in the dark for 250 min / revolution with magnetic stirring for 1 hour, and let stand at 4°C for 1 hour. Centrifuge at 12,000 rpm for 10 minutes, take the precipitate to obtain the product AE@ZIF-8 nanoparticles, resuspend in methanol, centrifuge again, repeat three times, and resuspend the final product in 5 ml methanol.

[0074] Dissolve 10mg COOH-PEG-PLGA in 4.5ml phosphate buffered saline (PBS), dissolve in 120W ultrasonic bath for 5 minutes, and then add 1mg / ml 1-e...

Embodiment 2

[0076] Embodiment 2. Drug-loaded ZIF-8 nanoparticles covered with TF-PEG-PLGA coating and preparation method thereof

[0077] Dissolve 75mg zinc nitrate in a 2.5ml sample bottle (bottle A), dissolve in a 120W ultrasonic bath for 2 minutes; take 165mg dimethylimidazole and 10mg aloe-emodin and dissolve them in 5ml methanol (bottle B), take a 120W ultrasonic bath for 5 minutes Dissolve and stir magnetically for 5 minutes at room temperature, 250 rpm. Add bottle A to bottle B dropwise, and at room temperature, keep in the dark for 250 min / revolution with magnetic stirring for 1 hour, and let stand at 4°C for 1 hour. Centrifuge at 12,000 rpm for 10 minutes, take the precipitate to obtain the product AE@ZIF-8 nanoparticles, resuspend in methanol, centrifuge again, repeat three times, and resuspend the final product in 5 ml methanol.

[0078] Dissolve 10mg COOH-PEG-PLGA in 4.5ml phosphate buffered saline (PBS), dissolve in 120W ultrasonic bath for 5 minutes, mix AE@ZIF-8 solution a...

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Abstract

The invention discloses a drug-loaded ZIF-8 nanoparticle covered with a TF-PEG-PLGA coating and a preparation method and application thereof.The preparation method comprises the steps that a target drug, 2-methylimidazole and zinc nitrate are added into a methanol solution to be mixed for a first reaction, then the target drug, 2-methylimidazole and zinc nitrate are purified and dissolved in methanol, and a drug-loaded ZIF-8 nanoparticle solution is obtained; activating COOH-PEG-PLGA, uniformly mixing the activated COOH-PEG-PLGA with transferrin in the PBS to carry out a second reaction, purifying and dissolving in the PBS to obtain a transferrin modified PEG-PLGA solution; and uniformly mixing the drug-loaded ZIF-8 nanoparticle solution with the transferrin modified PEG-PLGA solution, so as to obtain the drug-loaded ZIF-8 nanoparticles covered with the TF-PEG-PLGA coating. The nanoparticle has blood-brain barrier permeability and tumor targeting property, realizes targeted drug delivery for craniocerebral tumors, and provides an ideal carrier for efficient and targeted drug delivery of intracranial tumors.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to drug-loaded ZIF-8 nanoparticles covered with a TF-PEG-PLGA coating and a preparation method and application thereof. Background technique [0002] Glioma is the most common primary tumor in the human central nervous system, accounting for about 80% of all primary malignant brain tumors. Glioblastoma (GBM) is a grade IV glioma, accounting for about 80% of all primary malignant brain tumors. Glioma accounts for 54% of the total, and has the characteristics of high incidence, high recurrence, high mortality and low cure rate. Surgery combined with radiation therapy and chemotherapy is the main treatment for GBM. However, due to the invasive growth pattern of GBM, complete surgical resection is difficult, and radiotherapy has problems of radioresistance. The application of chemotherapeutic drugs can improve the prognosis of patients with surgery and radiotherapy, but t...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/10A61K47/42A61K47/34A61K47/69A61K47/54A61K31/122A61P35/00B82Y5/00B82Y40/00
CPCA61K9/5169A61K9/5153A61K9/5146A61K47/6949A61K47/545A61K31/122A61P35/00B82Y5/00B82Y40/00Y02A50/30
Inventor 方兴刚罗杰郭兴荣杜士明汪嫚柏中喜马石楠冯莹陈卓李童斐兰培敏陈汉玉鲁军体张宇强皇甫沁媛
Owner 十堰市太和医院(湖北医药学院附属医院)
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