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Oligopeptide FTLE separated from chilli seeds and application of oligopeptide FTLE in prevention or treatment of cancers

A pepper seed and oligopeptide technology, applied in the biological field, can solve the problems of high cost, safety, and little research on active peptides.

Active Publication Date: 2022-08-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physiologically active functions of bioactive peptides are mainly reflected in antibacterial, antiviral, antioxidative, antitumor, lowering blood sugar, lowering blood pressure, lowering cholesterol, immune regulation, etc. Animal source active peptides have problems such as high cost and safety, while Plant-derived active peptides are widely used because of their pure nature and high nutritional value, such as soybean active peptides, peanut active peptides, rapeseed active peptides, etc., but there are still few studies on active peptides in pepper seeds

Method used

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  • Oligopeptide FTLE separated from chilli seeds and application of oligopeptide FTLE in prevention or treatment of cancers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, the oligopeptide FTLE in pepper seeds was extracted according to the following method:

[0034] 1) Deseed: fresh chili seeds and flesh are separated to obtain chili seeds;

[0035] 2) Crushing: Grind the chili seeds into chili seed meal, pass through an 80-mesh sieve, and obtain chili seed powder;

[0036] 3) Degreasing: Mix capsicum seed meal and n-hexane at a ratio of 1:10 (g / ml), stir and degreasing overnight, and remove n-hexane by suction filtration after degreasing to obtain capsicum seed meal;

[0037]4) Extract protein: Dissolve the defatted pepper seed meal in water at 1:10 (w / v, unit g / mL), adjust the pH of the solution to 9.5 with NaOH solution, dissolve for 4 hours, and then adjust the solution with HCl The pH value was 4.5, precipitated for 2 h, the reaction solution was centrifuged at 8000 rpm for 20 min, and the precipitate was collected as crude protein extract;

[0038] 5) Ultra-high pressure-assisted enzymatic hydrolysis: After the p...

Embodiment 2

[0042] The peptide sequence obtained by mass spectrometry identification and analysis in Example 1 was chemically synthesized to obtain a synthetic peptide. The effect of each peptide on the proliferation of HepG2 cells was studied separately, and the specific steps were as follows:

[0043] 1) HepG2 cell culture: HepG2 cells are derived from ATCC cell bank, HepG2 cells are cultured in DMEM medium containing 10% FBS, and the growth environment is a 37°C, 5% carbon dioxide cell incubator. At 25cm 2 The cells were cultured in culture flasks, passaged when the cells were grown to a density of 70%-90%, and the cells were seeded in 96-well plates.

[0044] 2) Peptide fragment treatment: After culturing cells in a 96-well plate for 24 hours, aspirate the original DMEM medium in the wells, add DMEM containing peptide fragments to each well, and the peptide fragment concentrations are 0.1, 0.3, and 0.6 mM, and continue to culture for 24 hours. .

[0045] 3) Measure the cell prolife...

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Abstract

The invention provides oligopeptide FTLE separated from chilli seeds and application of the oligopeptide FTLE in prevention or treatment of cancers, and the oligopeptide is separated from the chilli seeds, has an anti-tumor effect, especially can effectively inhibit growth and metabolism of HepG2 cells, and has a good application prospect.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to the oligopeptide FTLE isolated from capsicum seeds and its application in the prevention or treatment of cancer. Background technique [0002] Capsicum, a herbaceous plant belonging to the Solanaceae family, is native to Mexico and later introduced to China. It is now mainly distributed in Sichuan, Yunnan, Guizhou, Hunan, Henan and other places. Chili is mainly composed of chili pulp and chili seeds. Chili seeds account for 30%-60% of the dry weight of chili peppers and are the main by-products in the processing of chili peppers. Chili seeds are rich in dietary fiber, fat, protein, minerals and vitamin E, etc., and have excellent nutritional and economic value. [0003] With the rapid development of my country's chili processing industry, chili has been used in food, medicine, chemical industry and other fields. In the food field, there are more and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/107C12P21/06C07K1/14A61K38/07A61P35/00A23L33/18
CPCC07K5/1016C12P21/06A61P35/00A23L33/18A61K38/00Y02P60/87C07K14/415C07K1/145
Inventor 赵靓王凤章廖小军饶雷王永涛
Owner CHINA AGRI UNIV
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