Catechol 1, 2-dioxygenase as well as coding group, preparation method and application thereof
A dioxygenase and catechol technology, applied in the biological field, can solve the problems of high enzyme production and limited application of enzyme preparations, and achieve the effect of overcoming poor environmental adaptability and improving microbial degradation rate
Pending Publication Date: 2022-08-05
ENVIRONMENTAL PROTECTION RES INST OF LIGHT IND +1
0 Cites 0 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Abstract
The invention is applicable to the technical field of biology, and provides catechol 1, 2-dioxygenase as well as a coding group, a preparation method and application thereof, the amino acid sequence of the catechol 1, 2-dioxygenase is as shown in SEQ ID NO.2 in a sequence table, and the preparation method comprises the following steps: carrying out double enzyme digestion on an amplification product of the coding gene of the catechol 1, 2-dioxygenase, and carrying out enzyme digestion on the amplification product of the coding gene of the catechol 1, 2-dioxygenase to obtain the catechol 1, 2-dioxygenase. Connecting to a cloning plasmid to obtain a recombinant cloning plasmid; electrically transferring the recombinant clone plasmid to the first strain to obtain a clone strain; carrying out double enzyme digestion treatment on the recombinant clone plasmid and the expression plasmid, and connecting enzyme digestion producing areas of the recombinant clone plasmid and the expression plasmid to obtain a recombinant expression plasmid; electrically transforming the recombinant expression plasmid onto a second strain to obtain an expression strain; and carrying out induced expression culture on the expression strain to obtain the catechol 1, 2-dioxygenase. The catechol 1, 2-dioxygenase can be used for improving the microbial degradation rate of heavy oil.
Application Domain
BacteriaMicroorganism based processes +5
Technology Topic
Enzyme digestionMolecular biology +10
Image
Examples
- Experimental program(7)
Example Embodiment
Example Embodiment
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.