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Application of clenbuterol, method for detecting drug concentration, kit and application

A drug concentration and kit technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large amount of use, difficulty in quantitative detection of external standard method, inaccurate quantitative results of blood drug concentration in human serum, etc., to achieve accurate Quantitative, cost-reducing effects

Inactive Publication Date: 2022-08-05
BEIJING BGI GBI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the isotopic internal standard method is used to detect the blood drug concentration in human serum, an isotopic internal standard compound is required. On the one hand, it is difficult to commercialize stable isotopic internal standard compounds due to the high cost or difficulty of synthesis. Therefore, there is no commercial isotope internal standard compound in the vast majority of blood drug concentration monitoring; on the other hand, when detecting the blood drug concentration in human serum, most of the blood drug Internal standard compounds, the use of isotope internal standard compounds in the process of blood drug concentration monitoring will be large, which will cause expensive costs for blood drug concentration monitoring
When the external standard method is used to quantitatively detect the blood drug concentration in human serum, sample purification is required in the early stage of the test. The sample purification requires the use of protein precipitation (PPT) to remove the protein in the serum, and then multi-step dilution before the sample can be injected for detection; however, Multi-step dilution will introduce large errors, which will lead to inaccurate quantitative results of blood drug concentration in human serum, and cause great difficulties to the quantitative detection of external standard method

Method used

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  • Application of clenbuterol, method for detecting drug concentration, kit and application
  • Application of clenbuterol, method for detecting drug concentration, kit and application
  • Application of clenbuterol, method for detecting drug concentration, kit and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0105] This preparation example provides a screening process for internal standard compounds that can replace the internal standard in LC-MS / MS detection.

[0106] Among them, the optional internal standard compounds are cefoperazone impurity A, loratadine, ropivacaine, letrozole, chlorzoxazone, indomethacin, flurbiprofen, apramycin sulfate, Polymyxin B sulfate, kanamycin sulfate, tilmicosin, clenbuterol.

[0107] The screening process of the above-mentioned internal standard compounds is as follows:

[0108] 1. Solution preparation

[0109] (1) Preparation of internal standard working solution

[0110] Precisely weigh 10.000 mg of the above-mentioned alternative internal standard compound standard into a glass sample bottle, add 50% methanol aqueous solution, and obtain an internal standard stock solution with a concentration of 1.000 mg / mL after complete dissolution. Take an appropriate amount of the internal standard stock solution and dilute it with methanol to prepare ...

Embodiment 1

[0153] This embodiment provides a method for detecting drug concentration in human serum based on LC-MS / MS. Wherein, the drug detected in this example is voriconazole, and the used surrogate internal standard compound is clenbuterol.

[0154] The precision and accuracy of high-value samples (6.28 μg / ml) and low-value samples (5.10 μg / ml) of known concentrations of drug were analyzed by simulating the errors caused by the sample processing operations.

[0155] The above-mentioned method for detecting drug concentration in human serum based on LC-MS / MS specifically comprises the following steps:

[0156] 1. Solution preparation

[0157] (1) Preparation of internal standard working solution

[0158] Precisely weigh 10.000 mg of Clenbuterol standard into a glass sample bottle, add 50% methanol aqueous solution, and obtain an internal standard stock solution with a concentration of 1.000 mg / mL after complete dissolution. Take an appropriate amount of the internal standard stock ...

Embodiment 2-8

[0212] Embodiments 2-8 respectively provide a method for detecting drug concentration in human serum based on LC-MS / MS. The difference between the above embodiment and Embodiment 1 is that the types of drugs detected are different, as shown in Table 5.

[0213] Table 5 Drug species detected in the methods provided in Examples 2-8

[0214]

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PUM

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Abstract

The invention relates to the technical field of blood concentration monitoring, in particular to application of clenbuterol, a method for detecting drug concentration, a kit and application of the clenbuterol. According to the detection method and the kit provided by the invention, by knowing the properties of the compounds, the compounds with stable ionization are selected as the internal standard to calibrate the deviation generated in sample extraction, HPLC (High Performance Liquid Chromatography) sample injection and chromatographic separation and detection, so that accurate quantification is realized; the problem that at present, internal standards of aminoglycoside, carbapenems, glycopeptides, triazoles and other compounds are lacked or isotope internal standards are expensive is solved, and the method can be used for monitoring the blood concentration of the aminoglycoside, carbapenems, glycopeptides, triazoles and other compounds.

Description

technical field [0001] The present application relates to the technical field of blood drug concentration monitoring, and more particularly, to the application of clenbuterol, a method for detecting drug concentration, a kit and its application. Background technique [0002] At present, the commonly used analytical methods for therapeutic drug monitoring (TDM) mainly include immunological methods, high performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (LC-MS / MS). Among them, the traditional analysis techniques of TDM are mainly based on immunological methods. Immunological methods have the advantages of high sensitivity, fast analysis speed, high degree of automation, and simple operation, and are suitable for the determination of large quantities of samples. However, due to the limitation of the types of drug antibodies, most TDM drugs do not have suitable antibody supply, nor can they simultaneously Determination of multiple drugs or me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04
CPCG01N30/02G01N30/04G01N2030/045
Inventor 章申燕李宁蒋旭刘檬
Owner BEIJING BGI GBI BIOTECH