Detection kit and detection method for erythromycin and degradation products in animal-derived food
A degradation product, erythromycin technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve simple operation, high sensitivity and resolution, and good results
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Embodiment 1
[0078] Example 1 Construction of standard curve
[0079] (1) Preparation of isotope internal standard solution:
[0080] use 13 C,d 3 - Erythromycin was used as internal standard, and 50% (v / v) acetonitrile-water solution was used as solvent to prepare a final concentration of 1 μg / mL internal standard solution;
[0081] (2) Preparation of standard sample solution:
[0082] Erythromycin, Erythromycin A enol ether, Dehydrated Erythromycin A and N-Demethylerythromycin A were used as standard , diluted to 1 mg / mL with 50% (v / v) acetonitrile-water solution, and used as a standard sample working solution for later use.
[0083] (3) Preparation of standard solution to be tested:
[0084] Each standard working solution was prepared into nine levels of mixed standard working solution of 0.1, 0.5, 1, 5, 10, 50, 100, 200 and 500 ng / mL, and the concentration of the internal standard in each mixed standard was 50 ng / mL. mL;
[0085] (4) Detection conditions:
[0086] The obtained ...
Embodiment 2
[0106] Example 2 Recovery and Precision Determination
[0107] (1) Preparation of isotope internal standard solution:
[0108] use 13 C,d 3 - Erythromycin was used as internal standard, and 50% (v / v) acetonitrile-water solution was used as solvent to prepare a final concentration of 1 μg / mL internal standard solution;
[0109] (2) Standard solution preparation:
[0110]Erythromycin, Erythromycin A enol ether, Dehydrated Erythromycin A and N-Demethylerythromycin A were used as standards , respectively, diluted to 1 mg / mL with 50% (v / v) acetonitrile-water solution, and used as standard sample working solution for later use.
[0111] (3) Sample pretreatment:
[0112] Weigh 3g of turbot fish meat samples, add 50μL of internal standard solution and mixed standard solutions of ultra-low, low, medium and high concentration levels (see Table 2 for specific concentrations), then add 20mL of solvent 1 (acetonitrile) and The mixed solution of solvent 2 (water) (1:1, v / v) was homoge...
Embodiment 3
[0122] A method for detecting erythromycin and degradation products after boiling and heat treatment of animal-derived food based on a kit, comprising the following steps:
[0123] (1) Internal standard solution preparation:
[0124] use 13 C,d 3 - Erythromycin was used as internal standard, and 50% (v / v) acetonitrile-water solution was used as solvent to prepare a final concentration of 1 μg / mL internal standard solution;
[0125] (2) Positive sample preparation:
[0126] Take fresh turbot fish meat and mix it with erythromycin solution, so that the content of erythromycin in each gram of fish meat is 1000ng;
[0127] (3) Sample heat treatment:
[0128] Weigh 5 g of turbot fish meat to make fish balls, and boil them in water for 3 min, 5 min, 7 min, 10 min and 15 min at 100 °C, respectively, and four parallel experiments are carried out in each group;
[0129] (4) Sample pretreatment:
[0130] To 4.0-4.4g (boiled for 3min, 5min, 7min, 10min, 15min, the fish meat quality...
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