Silk glue protein extraction process

Abstract

The invention relates to the field of silk processing, in particular to a silk glue protein extraction process which comprises the following steps: S1, removing silkworm chrysalis in silkworm cocoons; s2, cleaning the silkworm cocoons from which the silkworm chrysalis is removed in the step S1, and drying the cleaned silkworm cocoons; s3, cutting the silkworm cocoons dried in the step S2 into silkworm cocoon fragments; s4, the silkworm cocoon fragments in the step S3 are subjected to ammonia gas fumigation through fumigation equipment 1, and the fumigation time is 1 h; s5, the silkworm cocoon fragments fumigated in the S4 are poured into a degumming pool 2 and heated to 90-95 DEG C, a used degumming solution is a sodium carbonate solution, the concentration of the sodium carbonate solution is 2.5%, the bath ratio is 1: 50, the degumming time is 30 min, in the degumming process, a mixture in the degumming pool needs to be continuously stirred, filtering is conducted after 30 min, and the silkworm cocoon fragments are obtained; silk glue protein containing a sodium carbonate solution is obtained; s6, the silk glue protein containing the sodium carbonate solution obtained in the S5 is repeatedly washed with deionized water and filtered, and the sodium carbonate solution covering the silk glue protein is cleaned up.

Description

technical field [0001] The invention relates to the field of silk processing, in particular to a process for extracting silk sericin. Background technique [0002] Silk is mainly composed of two proteins, silk sericin and silk fibroin, collectively referred to as silk protein. Properties: Sericin is a globular protein, the secondary structure is mainly random coil, and the stability is poor. It is soluble in hot water, acid, alkali, protease and low-concentration sodium carbonate solutions; silk fibroin is a fibrous protein. It contains a large number of crystalline structures and has stable performance. It is soluble in high-concentration calcium chloride, calcium chloride-ethanol-water mixture and lithium bromide. Therefore, according to the solubility difference of silk protein in different solutions and practical application requirements, Select appropriate lysate for extraction treatment to obtain various fibroin with different structures and properties, which are used...

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Problems solved by technology

[0003]However, the extraction of sericin in the prior art is often not thorough, and the sericin in the silkworm cocoon cannot be completely extracted, and the extraction rate of sericin is very low , resulting in a waste of materials, even if there is an extraction method that can efficiently extract sericin, but due to the limitation of special conditions, the amount of cocoons used at one time is extremely limited, and the amount is very small, which leads to extremely high extraction efficiency. Low, for example, the application number is: CN201410828143.1 patent discloses a method for extracting silk protein, although it discloses fumigation with ammonia before degumming, it is recorded in the patent: "described in step two The silkworm cocoon fragments are fumigated with ammonia gas. Specifically, the silkworm cocoon fragments are spread on the screen, and ammonia gas is introduced into the airtight environment for fumigation. The amount of ammonia used is 0.5-0.8 kg ammonia; the mass fraction of sodium bicarbonate aqueous solution in the step 3 is 15-20%", thus it can be seen that when fumigation is carried out to silkworm cocoon fragments, silkworm cocoon fragments need to be tiled on the sieve, if the effect of fumigation is good, Only a thin layer can be laid flat, so the amount of cocoon fragments that are degummed each time is very small. Once the cocoon fragments on the screen are too thick, the cocoon fragments in the middle will not be able to be fumigated by ammonia gas at all. It can be seen that although fumigation with ammonia gas greatly improves the unwinding rate of silkworm cocoon fragments, and finally can greatly increase the extraction rate of silk gum protein, but this method greatly reduces work efficiency and cannot meet the needs of society. There is a large demand for silk keratin in the world, so under the premise of high extraction rate, there is an urgent need for a fumigation equipment with a large amount of fumigation at one time and good fumigation effect to achieve efficient extraction of silk keratin

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