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Application of LPIN3 protein or gene for coding LPIN3 protein as biomarker of acute kidney injury

A technology for LPIN3 and acute kidney injury, applied in the field of biomedicine

Pending Publication Date: 2022-08-09
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports at home and abroad on the specific role of LPIN3

Method used

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  • Application of LPIN3 protein or gene for coding LPIN3 protein as biomarker of acute kidney injury
  • Application of LPIN3 protein or gene for coding LPIN3 protein as biomarker of acute kidney injury
  • Application of LPIN3 protein or gene for coding LPIN3 protein as biomarker of acute kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Detection results of LPIN3 protein expression in peripheral blood of patients with acute kidney injury and normal people and detection of monocyte LPIN3 gene mRNA expression in peripheral blood

[0038] Subjects: clinically diagnosed patients with acute kidney injury and control subjects

[0039] (1) Extract 3-5ml of peripheral venous blood from the patient into a disposable blood collection tube.

[0040] (2) Transfer the blood sample to a 15 mL centrifuge tube, centrifuge at 2000 rpm / min for 20 minutes, and separate the serum from the whole blood sample.

[0041] (3) After completing the centrifugation, the supernatant was taken as the serum sample and stored in an ultra-low temperature refrigerator at -80°C for later use.

[0042] (4) When in use, remove the lysate and centrifuge briefly.

[0043] (5) Use ELISA kit (Fankewei, F9970-A) to detect the expression level of LPIN3, the specific experimental steps are as follows:

[0044] (5.1) Dilute the origi...

Embodiment 2

[0088] Example 2. Cell viability detection results of primary renal tubular epithelial cells of LPIN3 knockout mice after stimulation with cisplatin

[0089] Subject: Primary renal tubular epithelial cells from LPIN3 knockout mice

[0090] LPIN3 knockout mice were purchased from Guangzhou Saiye Biotechnology Co., Ltd. First isolate mouse primary renal tubular epithelial cells:

[0091] (1) After the mice were sacrificed by cervical dislocation, the kidneys were aseptically removed in an ultra-clean workbench, and the kidneys were washed 2-3 times.

[0092] (2) After removing the renal pedicle and capsule, the kidney tissue was cut with scissors, placed in a 50 ml centrifuge tube, and digested with 1 mL of 1 mg / ml type II collagenase for 20 minutes.

[0093] (3) Terminate digestion with 2 mL of F12 high-glucose medium containing 10% fetal bovine serum, add an equal amount of PBS phosphate buffer to dilute and blow evenly, and collect the liquid through 100-mesh, 80-mesh and 4...

Embodiment 3

[0106] Example 3. Apoptosis detection results of primary renal tubular epithelial cells of LPIN3 knockout mice stimulated by cisplatin

[0107] Subject: Primary renal tubular epithelial cells from LPIN3 knockout mice

[0108] (1) The cells were seeded into a 6-well plate, and a cisplatin working solution with a concentration of 20 μM was used to stimulate the cells for 18 h and 24 h according to the method of Example 2.

[0109] (2) After the stimulation time, the cells were washed twice with PBS, and 300ul of EDTA-free trypsinized cells were added to each well.

[0110] (3) After the digestion is complete, add 600 ul of F12 high-glucose medium containing 10% fetal bovine serum to stop the digestion reaction, mix by pipetting, and suck it into a 15 mL centrifuge tube.

[0111] (4) Centrifuge at 1000 rpm / min for 5 minutes, discard the supernatant, and wash the cell pellet with PBS.

[0112] (5) Add 500 μL of binding buffer to each well, mix by pipetting, and transfer to a flo...

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Abstract

The invention relates to an application of an LPIN3 protein or a gene for coding the LPIN3 protein as a biomarker of acute kidney injury, relates to an application of the LPIN3 protein in evaluating toxic and side effects of kidney, and belongs to the field of biological medicines. The invention provides a novel application of an LPIN3 protein or mRNA (messenger ribonucleic acid) for coding the LPIN3 protein or a gene for coding the LPIN3 protein in evaluating renal toxicity and side effects. The amino acid sequence of the LPIN3 protein is as shown in SEQ ID NO. 1. The sequence of the mRNA for coding the LPIN3 protein is as shown in SEQ ID NO. 2. And the CDS sequence of the gene for coding the LPIN3 protein is as shown in SEQ ID NO. 3. A large number of experiments prove that the reduction of the expression quantity of the LPIN3 shows that the possibility of renal toxicity and side effects after an individual uses cis-platinum or a contrast agent is higher, and vice versa. Therefore, the content of the LPIN3 protein can be directly used for evaluating the severity of renal toxicity and side effects after an individual uses cis-platinum or a contrast agent to a certain extent, so as to provide guidance for whether the individual uses the cis-platinum or the contrast agent or not.

Description

technical field [0001] The invention relates to the application of LPIN3 protein or a gene encoding LPIN3 protein as a biomarker of acute kidney injury, and belongs to the field of biomedicine. Background technique [0002] Acute kidney injury (AKI) is an acute and critical illness characterized by a rapid increase in creatinine in a short period of time and a rapid decline in renal function caused by various reasons. Sepsis, ischemia / reperfusion, and the use of nephrotoxic drugs can all lead to AKI, and drug-related renal injury caused by nephrotoxic drugs (such as cisplatin, contrast agents, nephrotoxic antibiotics, etc.) accounts for all AKI cases 25%, is one of the main causes of AKI. Since the complex pathogenesis of AKI is still poorly understood, effective prevention and treatment measures are still lacking, resulting in high mortality and poor prognosis. According to statistics, 1.7 million people die of AKI every year in the world, which is at the forefront of cri...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11G01N33/573
CPCC12Q1/6883G01N33/573C12Q2600/158C12Q2600/166G01N2800/347G01N2333/916
Inventor 项荣刘宇星范亮亮王芳赵美方
Owner CENT SOUTH UNIV
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