Fusion anticaries DNA vaccine and its preparation

Abstract

A DNA vaccine for fusional decayed tooth prevention is prepared from Esherichia coli JM109 / pGLUA-P CCTCC No.M201042 through PCR amplification of the plasmid pYNB13 carrying the gene gtfB of deformed streptococci GS-5 glucosyl transferase GTT-1 to obtain sequence GLU in glucosan binding region, cloning it to eukaryotic vector pCI to obtain eukaryotic expression plasmid pGLU, and linking it with the A-P fragment sequence in regions A and P of the surface protein PAc of deformed streptococci in pCIA-P plasmid. Its advantages are simple method, high safety, low cost, durable immune response and high effect.

Description

technical field [0001] The invention belongs to the technical field of oral preventive medicine, and in particular relates to a PAc and GTF s Preparation method of fusion anti-caries DNA vaccine. Background technique [0002] Caries is a kind of bacterial infectious disease. Many scholars have proved that the genus Mutans Streptococci is highly related to caries, among which Streptococcus mutans and Streptococcus sobribus are the most important pathogenic bacteria that cause human caries. Glucosyltransferases (glucosyltransferaesGTFs) and surface protein (surface protein antigen PAc) (also known as antigen B, I / II, IF, P1) are important cariogenic virulence factors of Streptococcus mutans. GTF s Synthesize glucan and mediate the dextran-dependent adhesion of Streptococcus mutans on the tooth surface, and PAc is involved in mediating the non-glucan-dependent adhesion of bacteria to the acquired membrane on the tooth surface. The alanine-rich A region...

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Problems solved by technology

[0004] However, there are many deficiencies in the above various anti-caries peptide vaccines. Firstly, the preparation and purification of these peptides is difficult, generally takes several weeks and is expensive, and special conditions are required for storage; secondly, peptide anti-caries vaccines stimulate the body to produce effective immunity. The ability to respond is poor, and it is often necessary to add various immune adjuvants that are harmful to the human body; peptide anti-caries vaccines, like other peptide vaccines, generally have a short duration of induction of specific immune responses, and repeated vaccinations are required to achieve satisfactory results In addition, peptide vaccines generally cannot induce immune responses in infants and young children.

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