Polylactic acid porous scaffold for tissue engineering and its preparation method
A porous scaffold and tissue engineering technology, applied in the field of preparation of polylactic acid porous scaffolds for tissue engineering, can solve the problem of small pore size of polymer porous scaffolds, and achieve the effects of no residue of impurities, simple process and high porosity
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Embodiment 1
[0030] Example 1: The effect of the concentration of polylactic acid on the performance of porous scaffolds
[0031] Poly L-lactic acid is dissolved in the mixed solvent of dioxane / water (volume ratio 87 / 13) at 80 ℃, and the weight-volume concentration of poly-lactic acid is respectively 4%, 5% and 6% (w / v), Pour into a glass mold with a diameter of 1.2 cm and a thickness of 8 cm, roughen at 25°C for 5 hours, freeze at -20°C for 1 hour, and then freeze-dry in a freeze dryer for 24 hours to obtain a poly-L-lactic acid three-dimensional porous scaffold . After the stent was brittle broken by liquid nitrogen, the scanning electron micrograph (SEM) of the cross-section is shown in Fig. 1 . In Fig. 1, the concentration of a polylactic acid solution is 4%; the concentration of b solution is 5%; the concentration of c solution is 6%. The effect of polylactic acid concentration on the pore size and porosity of porous scaffolds is shown in Table 1.
[0032] Polylactic acid ...
Embodiment 2
[0036] Example 2: Effect of roughening time on the performance of polylactic acid porous material
[0037] Dissolve poly L lactic acid in a mixed solvent of dioxane / water (volume ratio 87 / 13) at 80°C, the weight volume concentration (w / v) of poly lactic acid is 5%, pour it into a 5 cm diameter and 4 mm thick In a stainless steel mold, roughen at 25°C for 0-8 hours, freeze at -20°C for 1 hour, and then freeze-dry in a lyophilizer for 24 hours to obtain a poly-L-lactic acid three-dimensional porous scaffold. After the stent was brittle broken with liquid nitrogen, the scanning electron micrograph (SEM) of the cross-section is shown in Fig. 2 . In Figure 2, a is not roughened; b is roughened for 1 hour; c is roughened for 6 hours; d is roughened for 8 hours. The pore diameter of the obtained porous scaffold was measured by electron microscope photos, and the effect of roughening time on the pore diameter and porosity of the obtained polylactic acid porous scaffold is shown in Ta...
Embodiment 3
[0039] Example 3: Effect of roughening temperature on properties of polylactic acid porous material
[0040] Dissolve poly-L-lactic acid in a mixed solvent of dioxane / water volume ratio (87 / 13) at 80°C, the weight-volume concentration of poly-lactic acid is 5% (w / v), pour into a diameter of 1.2cm and a thickness of 8cm In a glass mold, roughen at 25°C, 28°C, 31°C, 34°C, and 37°C for 5 hours, freeze at -20°C for 1 hour, and then freeze-dry in a freeze dryer for 24 hours to obtain Poly-L-lactic acid three-dimensional porous scaffold. After the stent was brittle broken by liquid nitrogen, the scanning electron micrograph (SEM) of the cross-section is shown in Fig. 3 . In Figure 3, the roughening temperature of a is 25°C, the roughening temperature of b is 28°C, the roughening temperature of c is 31°C, the roughening temperature of d is 34°C, and the roughening temperature of e is 37°C. The pore size of the obtained porous scaffold was measured by electron microscope photos, and...
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