Biosensor for lactic acid in whole blood
A biosensor, blood lactic acid technology, applied in the field of biosensors, can solve the problems of interference of oxygen content changes, poor redox characteristics, poor anti-interference ability, etc., to improve enzyme activity, improve response sensitivity, and avoid blood The effect of a change in volume
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Embodiment 1
[0011] A whole blood lactate biosensor such as figure 1 As shown, a thick-film carbon electrode 2 and silver electrode lead 2a were made on a PVC base plate 1 by screen printing technology, a plastic film was covered around the carbon electrode 2 to form a cavity 3, and 2 μl of 5 mg was dripped into the cavity. / mlCMC solution, after the solution is dried in a 60°C oven, a CMC layer 4a is formed; 2 μl of the modified carbon nanotube suspension is added at a concentration of 2mg / ml, and after being dried in a 60°C oven, a carbon nanotube layer 4b is formed. Then add 2 μl of mixed solution, the composition of which is 500 activity units / ml of L-lactate oxidase, 50 mg / ml of potassium ferricyanide, 20 mg / ml of seaweed, pH value of 6.0, and concentration of 0.2M phosphate buffer solution. Form the mixed layer 4c after drying in the oven, and then cover the cavity 2 with a plastic film to determine the volume of the cavity 3, which is 2 μl. The plastic film here is the cavity cover ...
Embodiment 2
[0013] A whole blood lactate biosensor such as figure 1 As shown, a thick-film carbon electrode 2 and silver electrode lead 2a were made on a PVC base plate 1 by screen printing technology, a plastic film was covered around the carbon electrode 2 to form a cavity 3, and 2 μl of 20 mg was dripped into the cavity. Carboxymethyl cellulose solution per ml, after the solution is oven-dried at 60°C, a CMC layer 4a is formed; 2 μl of modified carbon nanotube suspension is added dropwise with a concentration of 6 mg / ml, and after oven-drying at 60°C, a carbon nanotube layer 4a is formed. Nanotube layer 4b, add 2μl of mixed solution, the ingredients are L-lactic acid oxidase 1000 activity units / ml, potassium ferricyanide 50mg / ml, bovine serum albumin 4mg / ml, pH value 7.0, the concentration is 0.4M phosphate buffer solution is dried in a drying oven to form a mixed layer 4c, and then a layer of plastic film is covered on the cavity 3 as the cavity cover 5, the volume of the cavity 3 is ...
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