Pest control agent comprising multiple micro-organism
A technology for controlling pests and diseases, used in biocides, animal repellents, plant growth regulators, etc., can solve the problems of unsatisfactory pest and disease control effects, narrow range of pest control and high total investment costs, etc. It is not easy to produce drug resistance, the cost of input is low, and the total cost of investment is low.
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Embodiment 1
[0191] 1. Selection of strains:
[0192] 1) The biocontrol microorganisms are Bacillus thuringiensis AS 1.785, ACCC 10016, 10061, Beauveria bassiana AS 3.470, Metarhizium aeruginosa ACCC 30124, Paecilomyces AS 3.2762 strains as production strains.
[0193] 2) Beneficial microorganisms are Azotobacter phusticus ACCC 10001, AS 1.178, Azotobacter venerlande AS 1.1005, Rhizobium soybean ACCC 15005, AS 1.174, Flavobacterium oryzae ACCC 10051, Bacillus megaterium ACCC 10008, AS 1.127, waxy Bacillus AS 1.182 and Bacillus colloidus ACCC 10012 strains were used as production strains.
[0194] 2. Use the aforementioned method to prepare slant culture medium, production medium and prepare the required bacterial liquid. Take 30 kg of Bacillus thuringiensis bacterial liquid, 20 kg of Beauveria bassiana bacterial liquid, 30 kg of Metarhizium anisopliae bacterial liquid, 5 kg of Paecilomyces bacterial liquid, 5 kg of nitrogen-fixing bacterial bacterial liquid, 5 kg of phosphorus bacterial b...
Embodiment 2
[0201] 1. Selection of strains:
[0202] 1) The bio-control microorganisms are Pseudomonas fluorescens AS 1.33, ACCC 10040, Streptomyces cereus AS4.4653, ACCC 40010, Streptomyces griseus AS 4.456, ACCC 40075, Streptomyces californica ACCC 40015, water-absorbing chain Mold AS 4.168, Qingfeng Streptomyces AS 4.1036, Streptomyces schizophrenia AS4.748, Bacillus thuringiensis AS 1.1754, ACCC 10028, 10033, Beauveria bassiana AS 3.470, Beauveria bassiana powder AS 3.4429, Metarhizium anisopliae ACCC 30114, Metarhizium anisopliae ACCC 30124, Metarhizium anisopliae ACCC 30187, Metarhizium anisopliae ACCC 30105, and Paecilomyces AS 3.3728 strains were used as production strains.
[0203]2) Beneficial microorganisms are Azotobacter phusticosa ACCC 10006, AS 1.222, Azotobacter venerlande ACCC 10088, Flavobacterium oryzae ACCC 10052, Bacillus megaterium AS 1.516, and Bacillus colioids AS 1.231 as production strains.
[0204] 2. Use the aforementioned method to prepare slant culture mediu...
Embodiment 3
[0211] 1. Selection of strains:
[0212] The biocontrol microorganisms are Metarhizium anisopliae ACCC 30198, Metarhizium anisopliae ACCC 30102, 30104, 30131, Metarhizium anisopliae ACCC 30200, Metarhizium anisopliae ACCC 30114, Metarhizium anisopliae ACCC 30124, Metarhizium anisopliae ACCC 30195, Metarhizium anisopliae ACCC 30105, Paecilomyces aureus ACCC 30190, CFCC 5079, Paecilomyces AS 3.3728, ACCC 30147, Bacillus thuringiensis AS 1.897, ACCC 10025, 10028, Streptomyces griseus ACCC 40075, Coccidioides B. bassiana AS 3.4270, ACCC 30107, 30113, B. bassiana AS 3.3475, B. bassiana powder AS3.4429 strains are used as production strains.
[0213] 2. Use the aforementioned method to prepare slant culture medium, production medium and prepare the required bacterial liquid. Take 30 kg of Metarhizium anisopliae bacteria liquid, 15 kg of Bacillus thuringiensis bacterial liquid, 5 kg of Beauveria bassiana bacterial liquid, 5 kg of Paecilomyces bacterial liquid, and 5 kg of Streptomyc...
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