Algal food for improving physical ability of athletes and its preparation method
A nutritious food, Arthrospira technology, applied in food preparation, food science, application, etc., can solve the problems of large humidity changes, failure to cultivate Cordyceps sinensis and larvae, etc., achieve easy digestion and absorption, and enhance antioxidant effect , the effect of restoring physical strength
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Embodiment 1
[0194] Example 1. Preparation of algae and fungi extracts
[0195] The steps are:
[0196] (1) Enzymatic hydrolysis of algae and fungi
[0197] Take 100g of Spirulina (Spirulina) and 150g of Cordyceps militaris, grind them into powder (pass through a 100-mesh sieve), mix well, add 845ml of deionized water, 150 units of protease (trypsin can be used in implementation), set 30 to 35 In a constant temperature water bath for 8-12 hours, take it out and add 180 units of papain, put it back in a constant temperature water bath for 8 to 12 hours, take it out and put it in a -18 to 20 °C refrigerator for quick freezing. When it freezes into ice, take it out and let it dissolve naturally at room temperature, and then quickly freeze and dissolve. This cycle is repeated three times. Put the frozen solution in a high-speed centrifuge (6000γ / 15 minutes) to separate, add pre-frozen 95% alcohol to the supernatant, stir and let it stand for 24 hours, a precipitate appears, recover the alcoh...
Embodiment 2
[0200] Embodiment 2, the preparation of fruit and vegetable total DNA
[0201]After washing, crushing and freezing the fruits and vegetables in sequence, place them in a high-speed tissue grinder, add pre-cooled Nad-SSC-EDTA-SDS buffer solution, grind for 5-30 minutes, pour into a container and add an equal volume of chloroform - Isoamyl alcohol solution, shake vigorously for 20 minutes to separate the protein, put it in a centrifuge (3000γ / 15 minutes) to absorb the aqueous solution containing nucleic acid in the upper layer, discard the debris in the middle layer, denature the protein in the lower layer, and recover it in chloroform for another use. Pour the absorbed nucleic acid aqueous solution into an Erlenmeyer flask and place it in a 72°C water bath for 3 to 4 minutes, quickly take it out after inactivating the DNase in the tissue, immediately cool to room temperature, and slowly add a 5mol / L sodium perchlorate solution (4 : 1, V / V), so that the final concentration of so...
Embodiment 3
[0202] Embodiment 3, the preparation of fruit and vegetable auxiliary material:
[0203] (1) Preparation of concentrated fruit and vegetable pulp:
[0204] According to the growing season of fruits and vegetables, select an appropriate amount of food, select, wash, and peel (NaOH) in turn; vegetables, etc. are not peeled, cut into small pieces (crushed), squeezed, and add 0.05mol / L acetic acid solution (about 1 / 2 of the weight of the whole material), boil for 5 minutes (soften), rinse with water, cool to 45-50°C, adjust the pH to 4.5 with citric acid, drain and squeeze the juice to obtain a cloudy liquid, adjust the pH to 4.5 with citric acid, Add 0.1% pectinase and mix evenly, enzymatically hydrolyze for 2-4 hours, homogenize through a homogenizer, concentrate, fill in jars, seal, and sterilize at 116°C, that is, liquid auxiliary materials, and store for future use.
[0205] (2) Preparation of fruit and vegetable solid concentrates:
[0206] The homogenized fruit and vegeta...
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