D-ribose fermentation process regulating method
A fermentation process and ribose technology, which is applied in the field of regulation of D-ribose fermentation process, can solve problems such as optimized control methods that have not yet emerged, and achieve the effects of shortened fermentation cycle, easy control, and enhanced repeatability
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Embodiment 1
[0067] Bacillus pumilus (B. pumilus) CGMCC 0845 was used. In 350L seed medium (pH 7.0) consisting of 2.0% sorbitol (w / w), 2.0% corn steep liquor, 0.1% yeast extract, 0.3% dipotassium hydrogen phosphate, 0.1% potassium dihydrogen phosphate, and 0.05% magnesium sulfate Medium, control the pressure of the seed tank to 1.0kg / cm 2 , Rotation speed 200 rpm, culture temperature 36 ℃, after 16 hours of culture, access is made of glucose 16.0% (w / w), corn steep liquor 2.6%, yeast extract 0.1%, ammonium sulfate 0.7%, manganese sulfate 0.005%, calcium carbonate In a 2.2% fermentation medium (pH 7.0) of 3200L, the stirring speed was controlled to be 180 rpm and the culture temperature was 36°C. In the early period of fermentation, it is controlled at pH 6.5-7.0 and dissolved oxygen 20-25% for 0-18 hours; in the middle and late fermentation period, it is controlled at pH 6.3-6.5 and dissolved oxygen 25-30%. 18 hours before fermentation, glucose consumption is slow, mainly in the growth stage o...
Embodiment 2
[0073] B. subtilis (B. subtilis) CGMCC 0846 was used. In 350L seed culture medium composed of glucose 2.0% (w / w), yeast extract 0.3%, corn steep liquor 0.05%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05% (pH 7.0) , Control the pressure of seed tank 1.0kg / cm 2 , Rotation speed 200 rpm, culture temperature 36 ℃, after 18 hours of culture, connect with glucose 16.0% (w / w), corn steep liquor 2.6%, ammonium sulfate 0.7%, manganese sulfate 0.005%, calcium carbonate 2.2% 3200L fermentation medium (pH 7.0). Control the stirring speed at 180 rpm, and the culture temperature at 36°C. In the early period of fermentation, 0-18 hours, control at pH 6.5-7.0, dissolved oxygen 18-22%; in the middle and late fermentation period, control at pH 6.0-6.2, dissolved oxygen 20-25%; at 36℃, 0.60kg / m 2Cultivation under aeration and stirring. 18 hours before fermentation, glucose consumption is slow, mainly in the growth stage of bacteria. After 18 hours...
Embodiment 3
[0079] Bacillus pumilus ATCC 31093 was used. In 350L seed culture medium composed of sorbitol 2.0%, corn steep liquor 2.0%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, tyrosine and phenylalanine each 100μg / ml (pH 7.0), Control seed tank pressure 1.0kg / cm 2 , Rotation speed 200 rpm, culture temperature 36 ℃, after 16 hours of culture, access is made of glucose 16.0% (w / w), dry yeast 1.2%, ammonium sulfate 0.6%, manganese sulfate 0.005%, calcium carbonate 2.2%, tryptophan Acid, tyrosine and phenylalanine prepared 50μg / ml 3200L fermentation medium (pH 7.0), using the same fermentation control conditions as in Example 1, after 33 hours of fermentation culture, the glucose in the fermentation broth decreased 2.5-3.0%, control the sugar concentration in the fermentation broth to be within the range of 2.5-3.0%, and start to feed sugar at a flow rate of 120 liters / hour, the sugar concentration of the feed is 40%, and the volume is 550L. After 48 hours of fer...
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