Immobilized biological additive and its preparation method and uses
A technology for biological additives and tobacco, which is applied in the fields of application, tobacco, and tobacco treatment. It can solve the problems of occupying a large warehouse, a backlog of funds, and the quality of smoking is not as good as that of natural alcoholized tobacco leaves, so as to reduce diffusion, organic polymer substances, and The effect of good development and application prospects
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Embodiment 1
[0023] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, ethyl stearate 0.5%, potassium tartrate 0.05 %, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.
Embodiment 2
[0025] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, behenyl alcohol stearate 0.5%, tartaric acid Potassium 0.05%, sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate added to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.
Embodiment 3
[0027] The immobilized biological additive is prepared according to the following material composition: tobacco leaf degrading protease 1%, amylase 5%, pectinase 3%, polysaccharide degrading enzyme 5%, cellulase 0.8%, triglyceride 0.5%, potassium tartrate 0.05%, Sodium alginate 0.8%, isoamyl alcohol 0.5%, buffer acetic acid-sodium acetate add up to 100%. The immobilization conditions are as follows: the stirring speed is 400-500 rpm, the immobilization time is 4 hours, and the temperature is 25°C.
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