Polysaccharide quantitative detecting method and system

A quantitative detection method, polysaccharide technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as poor sensitivity

Inactive Publication Date: 2006-08-23
SHANGHAI UNIV OF T C M
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity of neutral polysaccharides is poor when directly applied to the literature method.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polysaccharide quantitative detecting method and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The mensuration of embodiment 1 Ophiopogon japonicus polysaccharide

[0017] Ophiopogon japonicus polysaccharide is β-D-fructan with a molecular weight of about 5000.

[0018] 1.1. Device

[0019] same figure 1 . Mobile phase is water, flow rate: 0.4mL / min; acid solution flow rate: 0.1mL / min; alkali solution of derivatization reagent is NaOH solution of guanidine hydrochloride, flow rate: 0.3mL / min. Reactor 1 is composed of a heating device that can be heated up to 170°C and a 0.5mm×5m polytetrafluoroethylene reaction tube; Reactor 2 is composed of a heating device that can be heated up to 170°C and a 0.5mm×10m stainless steel reaction tube; cooling The tube is a 0.25mm×3m stainless steel tube. Measurement wavelength: excitation wavelength (Ex) = 310nm, emission wavelength (Em) = 430nm.

[0020] 1.2. Determination of method determination conditions

[0021] 1.2.1. Acid solution concentration

[0022] Consider trifluoroacetic acid (TFA) as a representative. The c...

Embodiment 2D

[0055] The mensuration of embodiment 2Dextran T40

[0056] Dextran T40 is an α-D-glucan with a molecular weight of about 40,000.

[0057] 1.1. Device

[0058] same figure 1 . Mobile phase is water, flow rate: 0.4mL / min; acid solution flow rate: 0.1mL / min; alkali solution of derivatization reagent is NaOH solution of guanidine hydrochloride, flow rate: 0.3mL / min. Reactor 1 is composed of a heating device that can be heated up to 170°C and a 0.5mm×5m polytetrafluoroethylene reaction tube; Reactor 2 is composed of a heating device that can be heated up to 170°C and a 0.5mm×10m stainless steel reaction tube; cooling The tube is a 0.25mm×3m stainless steel tube. Measurement wavelength: excitation wavelength (Ex) = 310nm, emission wavelength (Em) = 430nm.

[0059] 1.2. Determination of method determination conditions

[0060] 1.2.1. Acid solution concentration

[0061] Take hydrochloric acid as a representative for investigation. The concentration of NaOH is calculated by fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method and system of polysaccharide, quantitative test, which is that polysaccharide is hydrolysis after isolation through chromatographic column then reacts with fluorescence biochemical reagent, and water content of polysaccharide is acquired by fluorescence detector measuring fluorescence derivative created. The invention has increased sensitivity of detection greatly by making polysaccharide react with fluorescence biochemical reagent 6 after its hydrolysis, is suitable for quantitative test of water content of polysaccharide to various kinds, whose testing limitation can reach tens of ng.

Description

technical field [0001] The invention relates to the field of pharmaceutical analysis. Specifically, it relates to a polysaccharide quantitative detection method and system. Background technique [0002] At present, the methods used for the determination of polysaccharide content mainly include chromogenic method, isotope labeling method, immunological method, biological assay method, high performance liquid chromatography (HPLC)-differential detection method and evaporative light scattering detection method. The detection limit of chromogenic method is about 10 μg, and the specificity is poor. The detection limit of isotope labeling method, immunological method and bioassay method can be less than 10ng, but they all have the disadvantage of not being able to simultaneously measure metabolites. Isotope labeling is not suitable for pharmacokinetic studies in humans, and labeling may change the structure of polysaccharides. Metabolic fragments with antigenic determinants may...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N30/80G01N30/84
Inventor 林晓徐德生冯怡沈岚
Owner SHANGHAI UNIV OF T C M
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap