Process for extracting actinidiaceae proteinase

A technology of kiwi fruit protease and fruit juice, which is applied in the direction of hydrolytic enzymes, etc., can solve the problems that have not been found in research reports, etc., and achieve the effects of shortening the process cycle, high efficiency, and easy filtration

Inactive Publication Date: 2006-09-06
SHAANXI INST OF ZOOLOGY NORTHWEST INSTOF ENDANGERED ZOOLOGICAL SPECIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the research on the application of tea polyphenols in this area is still in its infancy, there are few reference examples, and the application range is limited to a few kinds of proteins.
As for the use of tea polyphenols to separate and extract actinidia protease, no research reports on this aspect have been found through data retrieval.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024]Example 1 Take 1L of fresh kiwi fruit juice, mix it with 800ml of 0.3% tea polyphenol aqueous solution prepared (the powdered tea polyphenol produced by Wuxi Lvbao Company), stir evenly, adjust the pH value of the mixed solution to 5.0, and mix it at room temperature After standing for more than 4 hours, flocculent aggregates will settle at the bottom of the container. The supernatant was discarded, the lower layer of the diluted enzyme paste was transferred to a Buchner funnel for suction filtration, the precipitate in the collection funnel was vacuum-dried, pulverized by a ball mill and sieved to obtain 14.6 grams of kiwi fruit protease crude enzyme dry product, the crude The enzyme yield was 1.46%.

example 2

[0025] Example 2 Take 1L of fresh kiwi fruit juice and 1L of homemade tea water (use 22g of Shanqing tea, add 1500ml of water, boil for 3 minutes, soak in heat for 20 minutes and filter to obtain tea water with a tea polyphenol content of about 0.15%. Usually tea polyphenols The dissolution rate is about 10%), mix, stir evenly, adjust the pH value of the mixed solution to 4.5, let it stand for precipitation at room temperature for more than 2 hours, discard the supernatant, filter the lower layer of the enzyme paste, and collect the precipitate Carried out vacuum drying, pulverized by ball mill and sieved to obtain 15.2 g of dry product of actinidia protease crude enzyme, and the yield of crude enzyme was 1.52%.

example 3

[0026] Example 3 Take 2L of fresh kiwi fruit juice, add 200g of salt (NaCl), stir and dissolve, and store it at room temperature for 1-3 days. Get 1.8L of homemade tea water in Example 2, mix it with kiwi fruit juice, adjust the pH value to 4.0, let it stand for precipitation at room temperature for 3 hours, discard the supernatant, and filter the diluted enzyme paste at the bottom under reduced pressure to obtain the crude kiwi fruit protease. Enzyme wet product 62.8g. Get wherein 31.4g carry out vacuum freeze-drying, then carry out ball milling and sieve, obtain kiwi fruit protease crude enzyme dry product 17.6g, its crude enzyme yield is 1.76%; Remaining 31.4g crude enzyme wet product, carry out ethanol purification, Add 800ml of 80% (V / V) ethanol solution to dissolve the crude enzyme, stir evenly, transfer to a centrifuge tube and centrifuge at a speed of 4000r / min for 15 minutes, remove the supernatant, collect the sediment at the bottom, and vacuum freeze Dry, grind wit...

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PUM

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Abstract

A method of extracting kiwi fruit proteinase is disclosed. The invention employs 0.1-0.5% green tea polyphenols solution as precipitator, the volume ratio of green tea polyphenols solution with kiwi fruit juice is 0.8-1.1:1. Compared with the prior arts, the invention has merit of high yield, simple process, short period, low cost, innocuity etc, which is suitable for industrialized production of kiwi fruit proteinase.

Description

technical field [0001] The invention belongs to the improvement of the method for separating and extracting kiwi protease from kiwi fruit. Background technique [0002] Kiwi is a fruit with high nutritional value, known as "V c "King of King", health fruit and space food. In addition to a large amount of Vc and various mineral elements, kiwifruit fruit also contains a large amount of kiwifruit protease. Kiwifruit protease is a plant protease whose active center contains sulfhydryl groups. It is used in medicine, food , beer production, cosmetics, biochemical reagents and other fields have a wide range of uses. [0003] China is a big producer of kiwifruit, with a cultivation history of two thousand years. In the past 20 years, the artificial planting area of ​​kiwifruit has expanded rapidly. At present, the total area of ​​the country is 870,000 mu, with an annual output of 330,000 tons of fresh fruit. The kiwifruit resources are very rich. Kiwi fruit is a climacteric ber...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50
Inventor 左玉萍马齐
Owner SHAANXI INST OF ZOOLOGY NORTHWEST INSTOF ENDANGERED ZOOLOGICAL SPECIES
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