Medicine composition for treating coronary heart disease and its prepn process, use and guality control method
A technology for coronary heart disease and drugs, applied in the field of pharmaceutical compositions for the treatment of coronary heart disease and its preparation, use and quality control, can solve the problems of drug side effects, unsatisfactory, unacceptable by patients, and long-term use
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Embodiment 1
[0072] Embodiment 1: Preparation method Take 1100 grams of Rhodiola rosea medicinal material, add 8 times the amount of water and boil for 1.5 hours, filter, add 6 times the amount of the dregs of the medicine, boil for 1.5 hours, filter, combine the two medicinal liquids, concentrate into an extract, The amount of the paste obtained is about 190-200g, and the relative density is 1.2-1.25 (measured at 60°C). Take 1 part of the extract and 1.25-1.35 parts of starch, mix well, dry below 80°C, pulverize, make granules with ethanol at an appropriate concentration, and dry. The whole grain is packed into capsules, and 1000 capsules are obtained, each weighing 0.38g, which is equivalent to 1.1g of crude drug.
Embodiment 2
[0073] Example 2: The embodiment of identification method takes 1 g of the capsule content obtained above, puts it in a flask, adds 30 ml of ethanol with a concentration of 95%, heats to reflux for 1 hour, filters, concentrates the filtrate to dryness, and adds 2 ml of ethanol to the residue to dissolve , as the test solution. In addition, take the salidroside reference substance and add ethanol to make a solution containing 1mg per 1ml, as the reference substance solution, according to the thin-layer chromatography ("The People's Republic of China Pharmacopoeia 1990 Edition Part One") test, absorb the above two 2 to 4 μl of each of these solutions were spotted on the same thin layer of silica gel G containing sodium carboxymethylcellulose as a binder, and ethyl acetate-methanol-formic acid (9:1:0.8) was used as a developer. Unfold, take out to dry, spray with 1% FeCl 3 -1% Potassium ferricyanide (1: 1) solution, check under sunlight, in the chromatogram of the test product, ...
Embodiment 3
[0074] Embodiment 3: the embodiment of content determination method Accurately weigh about 2g of the capsule content prepared above, put in a flask, add 30ml of ethanol, heat and reflux for 1 hour, filter, filter residue is washed three times with 20ml of ethanol, combine the filtrate and concentrate to as far as possible. Dry, add 5ml of water to the residue to dissolve, add to a polyamide column (80-120 mesh, 5g dry packing, φ1.5cm), elute with water, accurately collect 25ml of the eluate, and use it as the test solution. The reference substance solution and the test solution 5--10μl are injected into the liquid chromatograph, and the peak area is measured under the conditions described in the system suitability test, and the external standard method is used to calculate it.
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