Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compsn. and method for hemoglobin and cell analysis

A technology of hemoglobin and hemoglobin concentration, which is applied in the direction of analyzing materials, biomaterial analysis, biological testing, etc., and can solve problems such as lack and toxicity

Inactive Publication Date: 2001-05-09
COULTER INTERNATIONAL CORPORATION
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] As noted above, current hemoglobin measurement reagents and methods are either toxic or lack the ability to perform multiple diagnostic assays in the same automated step, such as hemoglobin determination and counts of white blood cells or differential counts of white blood cell subsets

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compsn. and method for hemoglobin and cell analysis
  • Compsn. and method for hemoglobin and cell analysis
  • Compsn. and method for hemoglobin and cell analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Prepare reagents of the following composition. Formula 1a tetrazole 5.0g tetradecyltrimethylammonium bromide 15.0g distilled water, adjusted to 1 liter of pH 2.77 Formula 1b triazole 10.0g dodecyltrimethylammonium chloride (50% solution) 35.0ml tetradecyltrimethylammonium bromide 3.5g distilled water, adjusted to 1 liter of pH 6.19 formula 1c quinaldixic acid 5.0g dodecyltrimethylammonium chloride (50% solution) 50.0ml distilled water, adjusted to 1 liter pH 2.55 formula 1d melamine 5.0g tetradecyltrimethylammonium bromide 3.6g dodecyltrimethylammonium chloride (50% solution) 36.0 ml of distilled water, adjusted to 1 liter of pH 4.55 formulation le tetrazole 5.0 g of Chemfac NB-104 (complex phosphate manufactured by Chemax Corporation) 30.0 g of distilled water, adjusted to 1 liter of pH 7.11

[0096] With 2500 μl of ISOTON _ II Dilute 11.6 μl of whole blood sample, then manually mix 403 μl of one of the above lysing agent compositions with the pre-diluted sample. A...

Embodiment 2

[0098] Prepare reagents of the following composition. Tetrazole 5.0g dodecyltrimethylammonium chloride (50% solution) 36.0ml tetradecylammonium bromide 3.6g distilled water 1 liter pH 2.73

[0099] 11.6 μl of whole blood samples were diluted with 2500 μl of blood thinner, then 403 μl of the above lysing agent composition were manually mixed with the pre-diluted samples. Absorption spectra of the samples were immediately measured on a Beekman DU7500 spectrophotometer. Five commercial blood thinners were used for hemoglobin determination, the COULTER _ ISOTON _ Ⅲ、COULTER _ ISOTON _ Ⅱ、Technicon H ·TM Systems RBC DIL (commercially available from Technicon Instruments), standard phosphate buffered saline and standard sodium chloride saline. The chromogen formed has an absorption maximum at about 540 nm and a shoulder at about 565 nm. When different diluents are used for the determination of hemoglobin, the maximum absorption of the chromogen is only slightly different, t...

Embodiment 3

[0101] Prepare reagents of the following composition. Recipe 3a tetrazole 5.0g tetradecyltrimethylammonium bromide 15.0g distilled water, adjust to 1 liter pH 12.06 Recipe 3b aniline-2-sulfonic acid 5.0g tetradecyltrimethylammonium bromide Ammonium 10.0g cetyltrimethylammonium bromide 4.0g distilled water 1 liter pH 1.67 formula 3c quinaldicic acid 5.0g dodecyltrimethylammonium chloride (50% solution) 40. 0ml distilled water 1 liter pH 2.58

[0102] About 70 blood samples (with a hemoglobin concentration of 6-17g / dL and a white blood cell count of 1,000 / μL-40,000 / μL) were collected on a calibrated COULTER _ On the STKS instrument under standard instrument settings (except solvent, LYSE S _ Ⅲ diff is replaced by the above preparation) analysis. For each formulation, approximately half of the samples were clinical samples for various diseases. In the case of formulation 3a, 70% of the samples were clinical samples. Figure 5 shows that by using the reference reagent (LYSE ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

A cyanide-free lytic reagent composition and method for measuring the total hemoglobin concentration in a blood sample, for counting the number of leukocytes and for deferential counting of leukocyte subpopulations are described. The cyanide-free lytic reagent composition comprises a quaternary ammonium salt or a pyridinium salt to lyse erythrocytes and release hemoglobin, and an organic ligand selected from the group consisting of triazole and its derivatives, tetrazole and its derivatives, alkaline metal salts of oxonic acid, melamine, aniline-2-sulfonic acid, quinaldic acid, 2-amino-1,3,4-thiadiazole, triazine and its derivatives, urazole, DL-pipecolinic acid, isonicotinamide, anthranilonitrile, 6-aza-2-thiothymine, 3-(2-thienyl)acrylic acid, benzoic acid and alkali metal and ammonium salts of benzoic acid, and pyrazine and its derivatives to form a stable chromogen with hemoglobin, and a salt to adjust conductivity of the reagent for impedance measurement. The reagent composition is mixed with a blood sample without pre-dilution and the UV absorption of the sample mixture is measured at the predetermined absorption wavelength. Counting the number of leukocytes and differential counting of leukocyte subpopulations are accomplished simultaneously on an automated cell counter utilizing DC impedance measurement.

Description

[0001] This application is a continuation-in-part of co-pending US Application Serial No. 08 / 786,505 filed January 21,1997. Background of the invention [0002] Technical Field of the Invention [0003] The invention relates to a dissolving agent composition, a diluent and a method for manually or automatically measuring the total hemoglobin concentration in a blood sample and simultaneously performing leukocyte counting or differential counting of leukocyte subsets. [0004] discussion of prior art [0005] The measurement of total hemoglobin is an indicator of the oxygen-carrying capacity of whole blood. The ability to measure hemoglobin (Hgb) in a blood sample is a very important part of diagnostic assays and is also important in monitoring response to therapies for diseases that affect hemoglobin, as well as to therapies for other diseases that may adversely affect hemoglobin levels . [0006] Leukocytes in the peripheral blood of normal people are composed of five type...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/48G01N33/49G01N33/50G01N33/569G01N33/72
CPCG01N33/56972Y10T436/101666Y10T436/10Y10T436/105831G01N33/5094G01N33/721Y10T436/108331Y10T436/107497Y10T436/25125
Inventor 李毅C·杨
Owner COULTER INTERNATIONAL CORPORATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com