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Process for producing Lycopen

A technology of lycopene and products, applied in the field of separation and purification of lycopene, can solve the problem of not describing high-purity lycopene crystals and the like

Inactive Publication Date: 2002-09-18
VITATENE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A similar process for the preparation of highly pure β-carotene crystals by elution with water, low molecular weight alcohols or acetone has recently been described in International Application WO 98 / 03480, but no similar process has been described for the production of highly pure lycopene Application of prime crystal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Three liters of medium were harvested and the biosynthetic pathway was interrupted at the lycopene level. The concentration of the medium is 1.4 g lycopene per liter. The biomass in the culture medium was recovered by filtration in a Buchner funnel (a circular filter paper supported by a magnetic funnel or cardboard was used as a filter membrane) to obtain 880 g of wet biomass. The wet biomass was resuspended in 5.2 liters of constant boiling isopropanol 85 / 15 and stirred for 30 minutes. The purified biomass is recovered in a Buchner funnel.

[0054] The biomass was vacuum dried at less than 45°C for 18 hours until the residual solvent content was 1-2%. In this way, 150 grams of dry pure biomass is obtained, the lycopene content of which is equivalent to a concentration of 2.75%.

[0055] The dried biomass is ground and conditioned by a hammer mill with a 1 mm slit, so that the solid obtained has the same specific strength and can be extracted with a solvent.

[0056] Mix 1...

Embodiment 2

[0058] Cultivate 500 liters of fermentation medium and mix it directly with 500 liters of azeotropic isopropanol (isopropanol / water 85 / 15). After stirring for 30 minutes, the biomass was separated from the liquid by filtration under filter pressure. Approximately 130 kg of wet purified biomass can be collected.

[0059] The biomass was vacuum dried in an oven for 18 hours until the residual solvent content was 1-2%. The drying temperature is lower than 45°C and the time is 12-24 hours. In this way, 30 kg of biomass is obtained, and its lycopene content is equivalent to a specific concentration of 3%. Although its purity is slightly lower, it is actually the same as Example 1.

[0060] The dried biomass is ground and conditioned by a hammer mill with a 1 mm slit, so that the solid obtained has the same specific concentration and can be extracted with a solvent.

[0061] Mix 30 kg of ground solids with 350 liters of isobutyl acetate at 70°C and stir for 15 minutes. The treated bioma...

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PUM

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Abstract

Process of obtaining lycopene. The present invention relates to a new process of obtaining carotenoids, especially lycopene, starting from natural biosynthesis sources in general and, specifically, from submerged cultures of mucorales fungi such as Blakeslea, Choanephora or Phycomyces. The process described makes it possible to simplify the recovery process and obtain an increase in purity of the product. It comprises the following steps: 1) Direct treatment with alcohol on the natural source of biosynthesis (fermentation medium for example) and separation of a wet, purified biomass. 2) Conditioning of the purified biomass by drying plus disintegration or breakdown of the same. 3) Solid-liquid extraction of the lycopene with an organic solvent. 4) Concentration of the enriched extract. 5) Precipitation / crystallization by addition of alcohol. 6) Filtration. 7) Dryingin

Description

Invention field [0001] The present invention relates to a new method for separating and purifying lycopene. The lycopene is in principle derived from any natural raw material that is biosynthesized, more specifically from the deep culture of fungi of the order Mucor, such as Blaleslea, Choanephora or Phycomyces It started as a crystalline form for use by the human body. The present invention describes an extraction method that may simplify the recovery process and increase the purity of the products involved in the foregoing method. Background technique [0002] Carotenoid pigments are widely found in fruits and vegetables in nature, and give many substances in nature such as carrots, peppers, tomatoes, flowers or certain microorganisms the characteristic color from yellow to dark red. They can be divided into two categories, pure carbohydrates, such as carotene, including compounds such as β-carotene, α-carotene or lycopene; and carotene, which has antioxidant properties. Exampl...

Claims

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Application Information

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IPC IPC(8): A61K31/01A61K47/22A61K47/44A61P39/06C12P23/00
CPCC12P23/00Y02P20/582A61P39/06Y02E50/30
Inventor 安东尼奥·埃斯特雷利亚·德卡斯特罗阿尔方萨·J·科拉多斯·德拉·别哈埃尔曼诺·贝尔纳斯科尼曼努埃尔·埃斯特万·莫拉莱斯埃米利亚诺·冈萨雷斯·德普拉多
Owner VITATENE
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