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Protein chip for detecting infection disease in taken blood in blood bank

A protein chip and infectious disease technology, applied in the field of life sciences, can solve the problems of wasting manpower and material resources, different methods, and low efficiency, and achieve the effects of improving speed and efficiency, strong practicability, and safe operation

Inactive Publication Date: 2002-10-09
GENETECH BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such 4 items are tested one by one, the methods are different, repeated operations, low efficiency, long cycle, high cost, waste of manpower and material resources, especially not suitable for large-scale blood testing

Method used

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  • Protein chip for detecting infection disease in taken blood in blood bank
  • Protein chip for detecting infection disease in taken blood in blood bank
  • Protein chip for detecting infection disease in taken blood in blood bank

Examples

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Embodiment Construction

[0029] The blood bank blood collection infectious disease detection protein chip of the invention is to detect infectious diseases before blood collection from the blood bank, including antigens or antibodies corresponding to various blood detection indicators of hepatitis B, hepatitis C, AIDS and syphilis, as well as positive controls, negative controls, etc. The protein sample is dotted on the solid phase carrier at the same time, and the protein dotted on the solid phase carrier includes: hepatitis B surface antibody I, positive control and negative control, the positive control is hepatitis B surface antigen, and the negative control is human Serum albumin (HSA); hepatitis C antigen fragment I, positive control and negative control, the positive control is hepatitis C antibody, and the negative control is HSA; type I HIV antigen fragment I, positive control and negative control, all The positive control is type I HIV antibody, and the negative control is HSA; type II HIV an...

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Abstract

A protein chip for testing the infectious diseases from the taken blood in blood bank, its preparing process and its testing method are disclosed. The antibodies or antigens and positive and negativereferences of heapatitis B, hepatitis C, AIDS and syphilis are densely arrayed on solid carrier, to which the protein is linked via chemical bond. Said protein is arrayed in a cavity of multi-sample microarray biochip. The reaction results for more antibodies or antigens of different blood samples can be obtained by one-pass reaction. Its advantages are high sensitivity and high specificity.

Description

technical field [0001] The blood bank blood collection infectious disease detection protein chip of the invention belongs to the field of life sciences. A protein chip is a protein or polypeptide array arranged in an orderly manner on a solid substrate to realize parallel detection, identification, identification and diagnosis of proteins. It can be applied to biology, medicine and related fields. Background technique [0002] The spread of infectious diseases caused by blood transfusion has been increasing, and it is imminent to establish an accurate, fast and efficient blood collection and testing system for blood banks. The infectious disease items tested by blood bank blood collection mainly include: hepatitis B antigen, hepatitis C antibody, AIDS antibody and syphilis antibody. The corresponding traditional detection methods are: hepatitis B antigen, hepatitis C antibody detection: colloidal gold method, enzyme-linked immune reaction (ELISA) (...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/576G01N33/68
Inventor 张涛李宾彭永济李红梅任一萍
Owner GENETECH BIOTECH SHANGHAI
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