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Slow-releasing bFGF-PLA-PEG microball and its prepn and use

A microsphere and slow-release technology, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problems of no bFGF microspheres, and achieve improved drug release in vitro and particle size Distribution, the effect of improving the encapsulation rate

Inactive Publication Date: 2003-02-26
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after reviewing the literature, there is no report on bFGF microspheres and related experimental studies in China.

Method used

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  • Slow-releasing bFGF-PLA-PEG microball and its prepn and use
  • Slow-releasing bFGF-PLA-PEG microball and its prepn and use
  • Slow-releasing bFGF-PLA-PEG microball and its prepn and use

Examples

Experimental program
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Embodiment Construction

[0013] Accurately weigh 150 mg of PLA-PEG and other auxiliary materials and dissolve them in an organic solvent to form an oil phase. The organic solvent is dichloromethane (DCM) or a mixture of dichloromethane (DCM) and acetone (AC), and the volume percentage of DCM and AC is 75-100:0-35. 100 μg bFGF sample was dissolved in double distilled water to form the aqueous phase. The bFGF solution is added to the above oil phase, which forms colostrum after homogenization. Weigh an appropriate amount of PVA or / and PEG into double-distilled water, bathe in a 50°C water bath to fully dissolve, and adjust the concentration to 5%. Inorganic salts are added to the aqueous dispersion medium solution, so that the concentration of the outer aqueous phase is 2% or higher. Add the above emulsion dropwise to 2ml of PVA or PVA-PEG solution, homogenize again, then pour this solution into 8ml of PVA or PVA-PEG solution of the same concentration, and stir magnetically at room temperature for 3-5...

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Abstract

In the present invention the microsphere has coated bFGF as medicine, and matrix of lactic acid-glycol copolymer (PLA-PEG). The slow-releasing bFGF microsphere is prepared by using PVA-PEG mixed liquid as dispersing medium and through evaporation and mechanical stirring. It has excellent slow-releasing performance of over two-week releasing period. Its freeze dried powder has good dispersivity and low organic solvent residue. The present invention can be used for local administration or vein administration of treating fracture, bone defect and other diseases.

Description

technical field [0001] The invention relates to a slow-release microsphere of basic fibroblast growth factor (bFGF). The preparation method is an emulsion dispersion solvent evaporation method, the polymer material PLA-PEG is used as a carrier material to encapsulate bFGF, and the slow-release bFGF microsphere is prepared under the action of mechanical stirring. Background technique [0002] Since the 1980s, with the development of molecular biology and genetic engineering technology. On the basis of the traditional methods of treating wounds, humans have introduced genetic engineering products to promote wound repair, and cell growth factors are one of them. There are many types of cell growth factors, and their roles and mechanisms in wound repair are also quite different. Scholars at home and abroad have carried out a lot of research on this. Basic fibroblast growth factor (basic fibroblast growth factor, bFGF) is one of the research hotspots. bFGF is not only a mitoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K9/52A61K38/18A61P17/08
Inventor 裴福兴段宏陈坚沈彬王光林蒋学华黎峰
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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