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Method for batch-culturing bacillus subtilis variant strain by using solid fermentation

A technology of Bacillus subtilis and mutant strains, applied in bacteria and other directions, can solve the problems of loss of wet cell yield, large equipment investment, rising production cost, etc., and achieve the effect of reducing equipment investment and high yield

Inactive Publication Date: 2003-04-23
EAST CHINA NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method still has the following disadvantages: a special high-speed centrifuge is needed when harvesting the above-mentioned wet thalline, and the equipment investment is large; The yield loss of wet bacteria is large, resulting in an increase in production costs; the waste liquid after harvesting wet bacteria is difficult to treat, and it will cause new pollution when discharged into the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1. A method for cultivating Bacillus subtilis mutant strains in batches by solid fermentation, characterized in that the steps of operation:

[0015] The first step is to prepare solid medium, prepare solid medium for four solid fermentation tanks with a capacity of 500L, and put 120kg of solid raw materials into each tank, including soybean flour 36kg, corn flour 36kg, rice bran 24kg, bran 23.28 kg, ammonium sulfate 0.48kg and ammonium chloride 0.24kg, ammonium sulfate and ammonium chloride were dissolved in 42kg of water, injected into each tank, stirred evenly, and prepared into four tanks of the same solid medium;

[0016] In the second step of sterilization, the pressure, temperature and rotation speed of the fermenter are respectively 1.3kg / cm 2 , 125°C and 60rph, four tanks of solid medium were autoclaved for 55 minutes;

[0017] The third step is to inoculate and cultivate. When the tank temperature drops to 40°C, spray 42kg of sterile water into each ...

Embodiment 2

[0019] Example 2. A method for cultivating Bacillus subtilis mutant strains in batches by solid fermentation, characterized in that the steps of operation:

[0020] The first step is to prepare solid medium, prepare solid medium for four solid fermentation tanks with a capacity of 500L, and put 60kg of solid raw materials into each tank, including 3kg of soybean flour, 33kg of corn flour, 20.64kg of rice bran, and bran 3kg, 0.24kg of ammonium sulfate and 0.12kg of ammonium chloride, dissolve ammonium sulfate and ammonium chloride in 19.2kg of water, pour into each tank, stir evenly, and prepare four tanks of the same solid medium;

[0021] In the second step of sterilization, the pressure, temperature and rotation speed of the fermenter are respectively 1.05kg / cm 2 , 121°C and 6rph, four tanks of solid medium were autoclaved for 35 minutes;

[0022] The third step is to inoculate and cultivate. When the temperature of the tank drops to 40°C, spray 19.2kg of sterile water int...

Embodiment 3

[0024] Example 3. A method for cultivating Bacillus subtilis mutant strains in batches by solid fermentation, characterized in that the steps of operation:

[0025] The first step prepares solid medium, prepares solid medium for four solid fermentation tanks with a capacity of 500L, and puts into each tank 90kg of solid raw materials, including soybean flour 4.5kg, corn flour 49.5kg, rice bran 4.5kg, Bran 30.96kg, ammonium sulfate 0.36kg and ammonium chloride 0.18kg, ammonium sulfate and ammonium chloride were dissolved in 28.8kg of water, injected into each tank, stirred evenly, and prepared into four tanks of the same solid medium;

[0026] In the second step of sterilization, the pressure, temperature and rotation speed of the fermenter are respectively 1.05kg / cm 2 , 121°C and 30rph, four tanks of solid medium were autoclaved for 40 minutes;

[0027]The third step is to inoculate and cultivate. When the tank temperature drops to 40°C, spray 28.8kg of sterile water into ea...

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PUM

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Abstract

The method for batch culture of bacillus subtilis variant strain by using solid fermentation. Its operation includes four steps of preparing solid culture medium, sterilizing, inoculative culture andharvesting living bacteria. It can collect humid thallus, and has no need of special centrifuger, so that its equipment investment is low, and it has no environmental pollution problems.

Description

technical field [0001] The present invention relates to a method for cultivating Bacillus subtilis variant strains (Bucillus Subtilis sp.) in batches by solid fermentation. # ,2 # 、3 # and 4 # The method belongs to the technical field of biological material cultivation. Background technique [0002] The water in aquaculture ponds is mainly polluted by two types: a large amount of human domestic sewage, such as human and livestock excrement, washing water, etc., is discharged into rivers and lakes, causing the rivers and lakes to contain a large amount of ammonia nitrogen, nitro nitrogen and organic matter. The above pool water is drawn from the river , lake, the pool water also contains a large amount of ammonia nitrogen, nitro nitrogen and organic matter; the above pool water is also polluted by the residual feed and excrement of aquatic products, and the macromolecular organic compounds contained in the pollutants cause the eutrophication of the wat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 吴自荣郭大磊黄静
Owner EAST CHINA NORMAL UNIV