Method for preparing water-soluble nano particles

A nano-particle, water-soluble technology, applied in the interdisciplinary field of disciplines, can solve the problems of poor stability, easy agglomeration, and long time required for water-soluble nano-quantum dots, and achieve low cost, simple method, and wide application.

Inactive Publication Date: 2003-07-23
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some literatures have reported the method that nanoparticles are transferred from organic phase to aqueous phase, such as: Alivisatos et al. [document Bruchez M Jr.; Moronne M.; Gin P.; Weiss S.; , 281, 2013-2015] reported that after coating semiconductor nano-quantum dots with silylating reagents, these semiconductor nano-quantum dots can be dispersed in aqueous solution with good stability. The disadvantage of this method is that the operation is very complicated, The time required is long and the cost is high; Chan et al. [Document Chan W C W.; Nie S M.Science.1998, 281, 2016-2018] adopt a very simple method to coat semiconductor nano-quantum dots with thioglycolic acid, and prepare Water-soluble semiconductor nano-quantum dots have been obtained, however, the water-soluble nano-quantum dots prepared by this method have poor stability and are prone to agglomeration

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  • Method for preparing water-soluble nano particles
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  • Method for preparing water-soluble nano particles

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Embodiment 1

[0020] The technical scheme of the present invention is further described in conjunction with specific examples. Example 1: Water-soluble semiconductor nanometer quantum dots are used as copper ion selective probes

[0021] (1) Preparation of ZnS-coated CdSe core-shell quantum dots

[0022] Using CdO and Se powder as raw materials, CdSe quantum dots were prepared by the method in the literature. ZnS-coated CdSe core-shell quantum dots are synthesized by a new method we explored (this method has applied for a patent). The transmission electron microscope (TEM) image shows that the particle size distribution of the synthesized core-shell quantum dots is uniform, and the diameter is about 5nm. The quantum yield of quantum dots synthesized in different batches is between 60% and 85%.

[0023] (2) Preparation of bovine serum albumin (BSA)-coated water-soluble quantum dots

[0024] The ZnS-coated CdSe core-shell quantum dots dispersed in n-hexane were precipitated with methanol, ...

Embodiment 2

[0027] Nano Fe dispersed in n-Hexane 3 o 4 Synthesized according to the method in the literature [Sun S H. Zeng H. J. Am. Chem Soc. 2002, 124: 8204-8205]. Nano-Fe dispersed in n-hexane 3 o 4 Precipitate with ethanol, and wash with ethanol 2-3 times to remove excess organic molecules on the surface, take about 2mg of nano-Fe 3 o 4Re-disperse in 0.5mL n-hexane solution, then put about 50mg bovine serum albumin (BSA) and n-hexane solution together into the mortar, moderately grind to mix evenly, add 4mL PBS after the n-hexane is completely volatilized (pH7.4) buffer solution to dissolve it, centrifuge the solution at a speed of 18000 rpm, discard the supernatant, remove excess BSA, wash the precipitate with a small amount of PBS buffer solution, and then redissolve in PBS buffer solution, Centrifuge at a speed of 5000 rpm to remove agglomerated nano-Fe 3 o 4 . The upper layer is water-soluble nano-Fe coated with BSA 3 o 4 solution. Figure 10 is the prepared water-solu...

Embodiment 3

[0029] Precipitate the CdSe / ZnS core-shell quantum dots dispersed in toluene with methanol, wash with methanol 2-3 times to remove excess organic molecules on the surface, dry them under the protection of Ar, take about 1mg and redisperse them in 0.5 mL of toluene solution, then put 5 mg of immunoglobulin (IgG) and the toluene solution of quantum dots into a mortar, grind them moderately to mix them evenly, and add 2 mL of PBS (pH7.4) buffer solution after the toluene is completely volatilized , to dissolve it, centrifuge the solution at 18000 rpm, discard the supernatant, remove excess immunoglobulin, wash the pellet with a small amount of PBS buffer solution, then redissolve in PBS buffer solution, and centrifuge at a lower rpm , to remove the agglomerated quantum dots. The supernatant is the water-soluble CdSe / ZnS solution coated with immunoglobulin (IgG).

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Abstract

A process for preparing water-soluble nano particles includes directly mixing the hydrophilic macromoleculae (protein, antibody, antigen, starch, etc) with the fluorescent or magnetic nano particles in non-polar or weak-polar solvent, mechanical grinding, volatilizing organic reagent, dissolving in water or buffering solution, and centrifugal separating twice. Its advantage is high speed.

Description

Technical field [0001] The invention relates to a method for preparing water-soluble nanoparticles, and belongs to the interdisciplinary field of materials, biology, physics, chemistry and other subjects. Background technique [0002] In recent years, nanoparticles have been widely used as probes in biology, physics, chemistry, medicine and other fields. Most of these systems are in aqueous solution. However, nanoprobes directly synthesized in aqueous solution usually have a wide size distribution, and some properties of the probes (such as fluorescence, magnetism, etc.) are not ideal. In order to solve these problems, people have developed the method of organic phase synthesis. Peng et al [documentation Peng Z A, PengX G J Am Chem Soc 2001, 123:183~184] with CdO, Cd(Ac) 2 CdSe semiconductor nano-quantum dots with uniform size and high quantum yield were synthesized using weak acid salts of cadmium as raw materials and using TOPO and other substances as solvents; on this ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/00B22F1/02
Inventor 何治柯梁建功谢海燕庞代文
Owner WUHAN UNIV
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