Recombinant human granulocyte-macrophage colony stimulating factor gel

[0006] An example is given below. Choose 350 grams of glycerol, 15 grams of carbomer, 10 grams of benzzet, 5 grams of sodium hydroxide, 10 grams of human albumin, and 10 undiluted solution 6 International unit, the rest uses purified water, a total of 1000 grams, the weight ratio of each component is: glycerol 35%, carbomer 1.5%, benzine 1%, sodium hydroxide 0.5%, human albumin 1%, Purified water is added to 100%. Among the ingredients of the formula, glycerol is used as a matrix, stabilizer, and protective agent; carbomer is used as a drug carrier; benzalze is used as a transdermal absorption enhancer; sodium hydroxide is used as a neutralizer to shape the gel and adjust the pH of the gel at the same time (PH), which keeps the original solution biologically active; human albumin acts as a stabilizer and protective agent. The biological product is manufactured under normal temperature conditions. The product process is as follows: Put 15 grams of carbomer in an emulsification tank, add 350 grams of glycerol, mix, stir, and disperse, and wait until the carbomer is dispersed in glycerol. After homogenization, add 10 grams of benzzet's purified aqueous solution, mix and stir. Carbomer absorbs water and swells. After the matrix is ​​evenly mixed, add 5 grams of purified aqueous solution of sodium hydroxide, mix and stir again, and the matrix gradually thickens to become Uniform, smooth, and delicate hydrogel matrix, milky white and translucent; finally add stock solution 10 6 A purified aqueous solution of international units and 10 grams of human albumin, reaching a total weight of 1000 grams, lightly mix and stir to form a recombinant human granulocyte macrophage colony stimulating factor gel with uniform texture, smoothness and good spreadability. The gel is mainly used for wound diseases such as skin and mucous membrane ulcers, scalds, burns, etc., and is directly applied to the affected parts.

[0007] Models of ulcer wound, burn wound and scald wound made after depilation on both sides of the body of healthy guinea pigs and healthy rabbits with the original solution, physiological saline, and matrix were randomly divided into groups, and the applicator comparison test was carried out on three and seven days. After inspection, the three kinds of wound reduction degree and the treatment effect of the animal group using this gel were significantly better than the original solution, normal saline and matrix group.