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Pyloric spirobacterium inhibitor

A kind of Helicobacter pylori, inhibition technology, applied in the field of isolates derived from Helicobacter erinaceus

Inactive Publication Date: 2003-09-10
上海中科伍佰豪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many reports suggest that Hericium erinaceus contains polysaccharides, alkaloids, terpenes and other biologically active ingredients, whether these ingredients have an inhibitory effect on Helicobacter pylori has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Extraction and purification of isolates with inhibitory effect on Helicobacter pylori

[0046] The fruiting body of Hericium erinaceus cultured in solid is used as raw material, according to figure 1 The process shown is for extraction. Wherein, the mixture of the crushed fruit body and 10 times of water was refluxed for 3 hours at 100° C., 5 times in total; the mixture of filter residue and 12 times of alkaline ethanol solution (pH10) was refluxed for 3 hours, 5 times in total; The mixture of acidic ethanol (pH 3-5) solution was refluxed 5 times for 3 hours.

[0047] Components FrI, FrII, FrIII and FrIV are prepared. The extraction rate of each component is shown in Table 1.

[0048] components

Embodiment 2

[0050] Further Separation of Component FrIII

[0051] A. Thin layer chromatography (TLC) and results

[0052] Thin-layer chromatography was carried out using the following systems as developing solvent and sulfuric acid: methanol (1:1) as developing reagent.

[0053] System 1—n-hexane:ethyl acetate (1:1)

[0054] System 2—n-hexane:ethyl acetate:ether (1:1:1)

[0055] System 3—chloroform:ether:ethyl acetate (9:1:1)

[0056] System 4—toluene: ethyl acetate: acetic acid (12:4:0.5)

[0057]The results showed that toluene: ethyl acetate: acetic acid (12:4:0.5) was the best developing solvent. There are about 10 spots in the ethanol extract of the fruiting bodies of Hericium erinaceus.

[0058] B. Silica gel column chromatography conditions and results

[0059] Silica gel: 200-300 mesh

[0060] Eluent: chloroform: methanol = 9:1, 8:2, 1:1

[0061] Developing agent: toluene: ethyl acetate: acetic acid = 12:4:0.5

[0062] Flow rate: rapid separation, 400ml / 15 minutes

[0063...

Embodiment 3

[0067] Qualitative identification of each component or fraction

[0068] (1) FrI and FrII:

[0069] A color development method using anthrone sulfuric acid. Take a small amount of the above precipitate, dissolve it in deionized water, take 1ml of the solution, add 4ml of anthrone sulfuric acid reagent (1g of anthrone dissolved in 500ml of concentrated sulfuric acid), boil for 10 minutes, it turns blue-green, which proves that it contains polysaccharides.

[0070] (2) FrIII

[0071] A. Using acetic anhydride-sulfuric acid reaction (Liebermann-Burchard reaction):

[0072] Take each 1ml of the concentrated solution in Example 1, evaporate to dryness, add 1ml of acetic anhydride to dissolve, then add 2 drops of concentrated sulfuric acid, and it turns yellow→purple→dark green, indicating that it contains saponins and triterpenes.

[0073] B. Utilize TLC

[0074] Carry out TLC by the method described in embodiment 2,

[0075] The results showed that in the FrIII component of t...

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Abstract

The present invention provides a kind of separated matter from hedgehog fungus and capable of being used as pyloric spirobacterium inhibitor. The pyloric spirobacterium inhibitor includes components of Rf value 0.15-0.6 extracted from hedgehog fungus sporophore and through alkali alcohol extraction and thin-layer chromatography. The present invention also provides the process of obtaining the separated matter and the pyloric spirobacterium inhibitor containing the matter. The separated matter and the inhibitor can inhibit the growth of pyloric spirobacterium effectively.

Description

technical field [0001] The present invention relates to isolates derived from Hericium erinaceus, more particularly, the present invention relates to isolates derived from Hericium erinaceus useful as inhibitors of Helicobacter pylori. Background technique [0002] Since Marshall and Warren isolated Helicobacter pylori (Hp) from gastritis patients in 1982, scientists from all over the world have conducted extensive research on the biological characteristics, pathogenic mechanism, diagnosis and treatment of Helicobacter pylori, and now It is confirmed that Helicobacter pylori is the main pathogenic factor of chronic gastritis and is closely related to the pathogenesis of peptic ulcer. WHO and International Cancer Research Organization have listed it as the first type of carcinogen. [0003] Clinically, it is difficult to eradicate Helicobacter pylori infection with a single antibiotic, so different drug combinations are used, such as bismuth-based dual and triple therapy. Al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61P1/04A61P31/04C07J9/00
Inventor 李平作谢松赵国屏陈常庆
Owner 上海中科伍佰豪生物工程有限公司