DNA vaccine pVPH for SARS virus

A technology of SARS virus and DNA vaccine, which is applied in the field of medical bioengineering, to achieve the effect of simple preparation process, strong effect and strong prevention of virus infection

Inactive Publication Date: 2003-10-22
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no specific drug against SARS virus, and it is imperative to develop an effective preventive vaccine

Method used

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  • DNA vaccine pVPH for SARS virus
  • DNA vaccine pVPH for SARS virus
  • DNA vaccine pVPH for SARS virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of SARS virus DNA vaccine pVPH

[0028] DNA vaccine pVPH structure see figure 1 , the construction process is shown in Figure 2.

[0029] 1. Synthetic HE-PDNA sequence (structure as described above), its 5' end contains Hind III restriction site 3' end contains Xho I restriction site

[0030] 2. Construct the recombinant plasmid of pVPH:

[0031] The HE-P DNA fragment was double digested with restriction endonucleases Hind III and Xho I. The reaction system is: HE-P DNA fragment 5μl (200ng / μl); 10×M Buffer (100mM Tris-Hcl pH7.5; 100mM MgCl 2 ; 10mM Dithionthreitol; 500mM NaCl) 2μl; Hind III enzyme (10U / μl) 1μl; Xho I enzyme (12U / μl) 1μl; 2 O to a total volume of 20 μl. Digest in a warm bath at 37°C for 2 hours. Afterwards, the digested samples were subjected to 1% low-melting point agarose gel electrophoresis, and the gel was cut to recover M-P fragments. At the same time, the pVAX1 vector was digested with Hind III and Xho I. ...

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Abstract

The present invention relates to the field of medicinal biological engineering technology, and is a DNA vaccine pVPH for preventing SARS virus. It is composed of human coronavirus HE protein antigenic determinant code sequence and signal peptide code sequence of plasminogen activator TPA to form secreted HE protein antigenic determinant code sequence HE-P, then inserting it into the eukaryotic expression vector. Said recombinant plasmid transformed competence colibacillus DH5 alpha is the pVPH engineering bacterium which can be used for preparing vaccine pVPH. Because its expressed antigenic protein can be secreted to the exterior of cell, so that it raises the immunity of body fluid, and can have the better action for preventing infection of SARS virus.

Description

Technical field: [0001] The invention relates to the technical field of medical bioengineering, and relates to a DNA vaccine pVPH for preventing severe acute respiratory syndrome SARS virus. Background technique: [0002] The World Health Organization referred to Severe Acute Respiratory Syndrome (SARS) as SARS, and confirmed that it is an infectious disease caused by the SARS virus (SARS coronavirus). At present, there is no specific drug for SARS virus, and it is imperative to develop an effective preventive vaccine. DNA vaccine is a new type of vaccine emerging in recent years, which has the advantages of long-lasting antibody titer and good cross-protection effect. In addition, the DNA vaccine also has the following outstanding advantages: (1) It can induce a full range of humoral and cellular immune responses and play a preventive role; (2) The immune protection time is long; (3) The production process is simple, the cost is low, the stability is good and the storage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61K48/00A61P11/00A61P31/14C12N15/50C12N15/79
Inventor 孙树汉郭瀛军黄力王开宇张术
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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