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Use of lysozyme and relative pure deriv. or raw liquid for preventing and treating diseases in sea and frech-water breeding

A freshwater aquaculture, lysozyme technology, applied in the directions of botanical equipment and methods, applications, biocides, etc., can solve problems such as difficult to treat antibiotics, infectious diseases, and antibiotics that are easy to induce drug-resistant bacteria.

Inactive Publication Date: 2003-10-29
长春奇龙生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, antibiotics remain in the fish, which affects the normal flora in the body of the person who eats them. A small amount of antibiotics can easily induce the emergence of drug-resistant bacteria. Therefore, many countries explicitly prohibit the import and sale of fish and meat with residual antibiotics; secondly, the long-term use of antibiotics , can induce pathogenic bacteria with drug-resistant bacteria in fish, and enter the human body with the food chain to cause infectious diseases, which are difficult to treat with antibiotics; finally, antibiotics will also affect the surrounding environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0007] Experimental example 1: In vivo bactericidal experiment of lysozyme 1. Materials 1. Strain: Vibrio alginolyticus 2. Animal: BALB / c mouse 2. Method

[0008] Vibrio alginolyticus infected BALB / c mice experiment will 10 5 ~10 9 Vibrio alginolyticus in the logarithmic growth phase of CFU (colony forming units) were inoculated intraperitoneally in 6-week-old BALB / c mice, and the mortality was calculated by observing the results.

[0009] The identification of lysozyme protection activity will be 1 × 10 9 Vibrio alginolyticus in the logarithmic growth phase was inoculated intraperitoneally on 6-week-old BALB / c mice as an infection control. The lysozyme protection group was injected with different doses of lysozyme at the same time as the bacteria were inoculated, and the results were observed for 7 days to calculate the protective power. Protection rate%=[(survival rate of experimental group-survival rate of control group) / mortality rate of control group]×100%. 3. Results...

experiment example 2

[0015] Experimental example 2: Anti-infection experiment of flounder protected by lysozyme 1. Materials 1. Bacterial strain: Vibrio vulnificus 2. Animal: Flounder 2. Method

[0016] Anti-infection experiment of protecting flounder fish with lysozyme The flounder fry were fed with lysozyme as an additive feed, and the normal feed was used as a control group. Will 1×10 9 Vibrio vulnificus in the logarithmic growth phase were inoculated intraperitoneally into the flounder fry of the protection group and the control group, respectively. Observe the results and calculate the protective power. Immune protection rate%=[(experimental group survival rate-control group survival rate) / control group death rate]×100% 3. Results

[0017] The results of the anti-infection protection experiment of lysozyme protection flounder fish showed that after the flounder fry were infected with Vibrio vulnificus, the protection rate of the flounder fry group fed with lysozyme as an additive could reac...

Embodiment 3

[0018] Example 3: Synergistic antibacterial effect of lysozyme and antibiotics 1. Materials 1. Strains: more than 400 clinical isolates, provided by Chengdu Antibiotic Industry Research Institute. 2. buffer solution pH7.2 10mM Tris-Cl buffer solution 3.LB culture medium 4. clarithromycin, roxithromycin 2. method Lysozyme is used in combination with clarithromycin and roxithromycin respectively (1:1),

[0019]Method 1 (test tube method) Use pH 7.2 10mM Tris-Cl buffer solution to make Staphylococcus aureus (or Escherichia coli) into a bacterial solution with a concentration of about 107-108 CFU / mL, and add it to different test tubes respectively, Add different doses of human lysozyme (100μg-1mg) to each 2mL test tube and overnight at 37°C; take 20μL from one of the tubes and dilute with 10-fold dilution method, apply 100μL on LB medium, and incubate overnight at 37°C , and observe the results, calculate the total number of bacteria in the original tube. After adding 100 μL of 1...

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Abstract

An use of the high-purity product or raw liquid of lysozyme and associated derivative in preventing and treating the diseases caused by vibrio, streptococcus, Edward bacterium, etc of the fish, crab,and shrimp cultured in seawater or fresh water is disclosed.

Description

Technical field: [0001] The invention relates to a lysozyme and its related derivative pure product or stock solution for the prevention and treatment of diseases of seawater and freshwater cultured fish, crabs and shrimps. Background technique: [0002] In recent years, my country's mariculture industry has developed very rapidly. It is estimated that the national mariculture fish water area is more than 1 million mu. According to the statistics of the Ministry of Agriculture, the output of my country's mariculture fish in 1995 was 150,000 tons. However, the occurrence of seawater fish disease has become a huge obstacle to the development of seawater cultured fish. Once the cultured fish is infected and popular, its mortality rate is generally 50-80%, or even no harvest. Moreover, after the fish farms are polluted by germs, they cannot be used again within a period of time. In addition, the pathogenic bacteria of some marine fish are zoonotic bacteria, such as Vibrio vul...

Claims

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Application Information

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IPC IPC(8): A01N63/00A61K38/48A61P31/04
Inventor 张华安米安献禄李元
Owner 长春奇龙生物技术研究所
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