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Biological testing strip

A test strip and biological technology, applied in the field of electrochemical analysis and clinical chemistry, can solve the problems of biological test strip sensitivity, linear response and anti-interference deterioration, easy loss of electron transfer mediators, short storage life of biological test strips, etc. , to achieve the effect of stable product size, high qualified rate of finished products, and prevention of loss

Inactive Publication Date: 2003-12-17
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the amount of enzyme used in the electrode is higher, thus increasing the cost of the biotest strip
Second, in the electrodes prepared by screen printing method, the electron transport mediators are easily lost, which makes the sensitivity, linear response and anti-interference of the biological test strips worse
Third, the storage life of this biological test strip is relatively short, and it usually needs to be refrigerated, which brings many difficulties and inconveniences to the transportation and storage of the product
In addition, the size of the electrode produced by screen printing technology is difficult to maintain stability, so the qualified rate of the product is low

Method used

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  • Biological testing strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 glucose test strip

[0029] 1) First, take 4×7cm 2 A ceramic sheet is used as the substrate, and titanium (50nm) and platinum (200nm) are vacuum-sputtered. Then use lift-off etching to make a platinum disk working electrode with a diameter of 2 mm, and a platinum arc reference electrode around the working electrode (see figure 1 ).

[0030] 2) Weigh 3.5 g of cyclodextrin (Shanghai Biochemical Reagent Co., Ltd.), add 50 ml of distilled water, and dissolve in a 60° C. water bath. While stirring, 50 ml of ferrocene ethanol (Tokyo Chemicals, Japan) solution (1.2 g / 100 ml) was added dropwise, and stirred in a water bath at 60°C for 4 hours. After cooling, it was separated by centrifugation, and the precipitate was taken out, washed three times with tetracyanofuran, washed three times with distilled water, and dried in vacuum.

[0031] 3) Dip a medical cotton swab with analytically pure acetone and gently scrub the electrode surface several...

Embodiment 2

[0039] Preparation of Example 2 Cholesterol Test Strips

[0040] The steps of Example 1 were repeated, except that in step 5), glucose oxidase was replaced by cholesterol oxidase (purchased from SIGMA, USA). The result is a test strip for testing cholesterol.

Embodiment 3

[0041] Embodiment 3 strip performance test

[0042] a) Test strip interchangeability

[0043] The 50 test strips prepared in Example 1 were taken as a batch, and 10 were randomly selected as a group, and the response current was measured in a 6.0 mmol / L glucose solution. After statistical processing, it can be concluded that the relative deviations measured by four batches of 17 groups of test strips are less than 10%. Meanwhile, the relative standard deviation of the test strip in serum was 7%. These results show that protein has no great influence on the interchangeability of the test strip, and the test strip prepared by the present invention is suitable for blood sample analysis.

[0044] b) Linear response of test strip

[0045] The test strip of Example 1 was tested in glucose solution and standard serum samples, and the results showed that it had a good linear response in the range of 0-16.0 mmol / L glucose concentration.

[0046] c) Life test

[0047] Several test ...

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Abstract

A biologic testing strip is composed of a basic strip, a working electrode and a counter electrode. On said working electrode there is the envelope substance prepared from cyclodextrin, bioactive enzyme, and electron transferring medium chosen from quinone, methyl blue, methyl violet, and ferrocene or its derivatives. Its advantages are low cost, and high exchangeability and anti-interference power.

Description

field of invention [0001] The invention relates to the fields of electrochemical analysis and clinical chemistry. More specifically, the present invention relates to a novel biological test strip and method for its preparation. Background technique [0002] Currently available commercially available biological test strips, such as blood glucose test strips for testing blood glucose levels, are usually produced by mixing bioactive enzymes (such as glucose dehydrogenase or glucose oxidase) with carbon paste, and then using screen printing technology. Test strips based on carbon paste electrodes (often called thick film electrodes). [0003] However, this carbon paste electrode has some disadvantages as follows. First, the amount of enzyme used in the electrodes is higher, thus increasing the cost of the biological test strip. Second, in the electrodes prepared by the screen-printing method, the electron transfer mediators are easily lost, thereby degrading the sensitivity, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/32G01N33/66
Inventor 王荣吴霞琴章宗穰朱建中张国雄
Owner SHANGHAI NORMAL UNIVERSITY
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