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Method for preparing low-molecular-weight chondroitin sulfate

A chondroitin sulfate, low-molecular-weight technology, which is used in medical preparations containing active ingredients, food preparation, pharmaceutical formulations, etc., can solve problems such as no chondroitin sulfate

Inactive Publication Date: 2004-01-28
汤毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no technology that can be used to produce low-molecular-weight chondroitin sulfate in my country, and there is no product with a molecular weight lower than 10,000 Daltons of chondroitin sulfate in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Put 1300 liters of deionized water in a jacketed reaction tank, heat to 65°C, add 30 kg of commercially available chondroitin sulfate (average molecular weight 29,000 Daltons) crude product, stir to dissolve, add 26 liters of 36% hydrochloric acid to the solution, add deionized Water to 1500 liters, the hydrochloric acid concentration of the solution is about 0.2N. After insulated and stirred for 10 hours, cool down rapidly, add 250 liters of 4% sodium hydroxide aqueous solution, make the pH value of the solution around 4.8, filter with an ultrafiltration membrane with a molecular weight cut-off (MWCO) of 4,000 Daltons, the molecular weight cut-off (MWCO) ) 2,000 Dalton nanofiltration membrane to concentrate, add 7 times the amount of 95% ethanol to the concentrated solution, crystallize, and dry to obtain 27.6 kg of white low molecular weight chondroitin sulfate (molecular weight 3,700).

Embodiment 2

[0024] Place 1300 liters of deionized water in a jacketed reaction tank, heat to 80°C, add 30 kg of commercially available chondroitin sulfate (average molecular weight 31,000 Daltons) crude product, stir and dissolve, add 13.5 kg of oxalic acid to the solution, and add deionized water to 1500 liters, so that the concentration of oxalic acid in the solution is about 0.1N. After being incubated and stirred for 12 hours, cool down rapidly, add 16 kilograms of calcium carbonate, separate the calcium oxalate precipitation, the pH value of the solution is about 5.1, filter with an ultrafiltration membrane with a molecular weight cut-off (MWCO) of 8,000 Daltons, and the molecular weight cut-off ( MWCO) 2,000 Dalton nanofiltration membrane to concentrate, add 8 times the amount of 95% ethanol to the concentrate, crystallize and dry to obtain 22.8 kg of white low molecular weight chondroitin sulfate (molecular weight 6,300).

Embodiment 3

[0026] Place 650 liters of deionized water in a jacketed reaction tank, heat to 73°C, add 40 kg of commercially available chondroitin sulfate (average molecular weight 31,000 Daltons) crude product, stir and dissolve, add 11.2 liters of 36% hydrochloric acid to the solution, and make the solution The concentration of hydrochloric acid is about 0.2N. After insulated and stirred for 8.5 hours, cool down rapidly, add 6.8 kg of sodium carbonate to make the pH of the solution around 4.6, filter with an ultrafiltration membrane with a molecular weight cut-off (MWCO) of 6,000 Daltons, and a molecular weight cut-off (MWCO) of 2,000 Daltons Concentrate with a nanofiltration membrane, add 7 times the amount of 95% ethanol to the concentrated solution, crystallize, and dry to obtain 37 kg of white low molecular weight chondroitin sulfate (molecular weight 4,400).

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Abstract

The preparation method of low molecular weight chondroitin sulfate is characterized by that it utilizes animal soft bone and / or marketable general macromolecular chondroitin sulfate as raw material, and adopts the conventional production process and the special processes of acid hydrolysis, ultrafiltration with ultrafiltration membrane and concentration to obtain the chondroitin sulfate whose molecular weight is less than 1000 daltons. Said product features high purity, good biological activity, high internal biological utilization rate and strong pharmacologic action, etc. and can be used asimportant raw material of medicine product, health-care product, cosmetics and food.

Description

[technical field] [0001] The invention relates to a preparation method of low molecular weight chondroitin sulfate. [Background technique] [0002] Chondroitin Sulfate is a macromolecular acidic mucopolysaccharide product made from pig, cattle, sheep and other animal cartilage tissue and aquatic product waste such as shark cartilage as raw materials by using biochemical extraction technology. Its sodium salt, potassium salt, calcium salt, zinc salt, etc. are widely used in medical and health products, food, cosmetics, pet feed, etc. As a blood lipid-lowering drug, chondroitin sulfate tablets are clinically used to treat hyperlipidemia. Chondroitin sulfate also has a variety of physiological functions and plays an important role in maintaining joint function and immune function. It is used to prevent and treat coronary heart disease, treat neuropathic pain such as migraine, and improve senile joint function degeneration. [0003] There are three main production methods of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163A23L33/10A61K31/737A61P3/06C08B37/08
Inventor 汤毅
Owner 汤毅
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