Method fo preparing medium, low molecular weight chondroitin sulphate and obtained product

A chondroitin sulfate, low molecular weight technology, applied in the field of preparation of medium and low molecular weight chondroitin sulfate, to achieve the effect of high purity

Inactive Publication Date: 2004-07-21
汤毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0026] So far, there is no method suitable for the preparation of medium and low molecular weight chondroitin sulfate, which can adjust the molecular weight as desired and fully guarantee the quality and purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of molecular weight chondroitin sulfate in embodiment 1

[0053] Put 4.5 liters of deionized water in a glass beaker, heat it to 70°C in a water bath, add 250 grams of commercially available chondroitin sulfate (average molecular weight 48,000 Daltons), stir and dissolve, add 145 ml of 36% hydrochloric acid to the solution, add deionized Water to 5 liters, so that the hydrochloric acidity of the solution is about 0.3N. After insulated and stirred for 6 hours, cool down rapidly, add 10% sodium hydroxide aqueous solution to make the pH of the solution around 10.5, and then use a centrifuge to remove the precipitate of miscellaneous proteins. The resulting supernatant was subjected to three-stage ultrafiltration with an ultrafiltration element with a molecular weight cut-off of 10,000 Daltons (polysulfone hollow fiber ultrafiltration membrane, Shanghai Yiming Filtration Technology Co., Ltd.), and then with a nanofiltration element (Hangzhou Water, St...

Embodiment 2

[0054] The preparation of molecular weight chondroitin sulfate in embodiment 2

[0055] Put 4.5 liters of deionized water in a glass, heat it in a water bath to 80°C, add 300 grams of commercially available chondroitin sulfate (average molecular weight 48,000 Daltons) crude product, stir to dissolve, add 45 grams of oxalic acid to the solution, and add deionized water to 5 liters, so that the concentration of oxalic acid in the solution is about 0.1N. After heat preservation and stirring for 8 hours, cool down rapidly, add 50 grams of calcium carbonate to make the pH of the solution around 9.5, and then use a centrifuge to remove the precipitates of foreign proteins and calcium oxalate. The resulting supernatant is filtered with an ultrafiltration membrane with a molecular weight cut-off of 20,000 Daltons, and the nanofiltration membrane is concentrated. The concentrated solution is crystallized by adding 95% ethanol, and vacuum-dried at 60° C. to obtain 276.8 grams of ...

Embodiment 3

[0056] The preparation of molecular weight chondroitin sulfate in embodiment 3

[0057] Put 650 liters of deionized water in a jacketed reaction tank, heat to 73°C, add 40 kg of commercially available crude product of common molecular weight chondroitin sulfate (average molecular weight 48,000 Daltons), stir and dissolve, add 11.2 liters of 36% hydrochloric acid to the solution, The hydrochloric acid concentration of the solution is about 0.2N. After insulated and stirred for 4 hours, cool down rapidly, add 7.0 kg of sodium carbonate to make the pH of the solution around 10.5, and then use a continuous flow centrifuge to remove the precipitate of miscellaneous proteins. The resulting supernatant is filtered with an ultrafiltration membrane with a molecular weight cut-off of 20,000 Daltons, the nanofiltration membrane is concentrated, and the concentrated solution is spray-dried to obtain 37 kilograms of white medium molecular weight chondroitin sulfate (average molecula...

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Abstract

A medium (or low)-molecular sulfate chondroitin is prepared through acid hydrolysis, alkali neutralization, separation, ultrafiltering, nanofiltering, and spray drying or crystallizing in alcohol. Its advnatages are high purity and easy absorption.

Description

technical field [0001] The present invention relates to a kind of preparation method of medium and low molecular weight chondroitin sulfate and the obtained product, specifically, the present invention relates to a kind of preparation method of medium and low molecular weight chondroitin sulfate between 2,000-30,000 Daltons and The resulting product. Background technique [0002] Chondroitin Sulfate is a macromolecular acidic mucopolysaccharide prepared from the cartilage tissue of pigs, cattle, sheep and other animals and the cartilage of aquatic products such as sharks by using biochemical extraction technology. Chondroitin sulfate sodium salt, potassium salt, calcium salt, zinc salt, etc. are widely used in medicine, health products, food, cosmetics, pet feed, etc. For example, chondroitin sulfate can be used as a blood lipid-lowering drug for clinical treatment of hyperlipidemia. Chondroitin Sulfate A Sodium Injection has the effects of lowering blood lipids, resisting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 汤毅
Owner 汤毅
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