Water soluble extract of red sage root and its preeparation method and use

A water-soluble extract, extract technology, applied in the field of medicine for diseases, to achieve the effect of good curative effect, sufficient drug source, and less toxic and side effects

Inactive Publication Date: 2004-10-06
CHENGDU DIAO PHARMA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the effect of salvianolic acid extract on the activity of angiotensin-converting enzyme.

Method used

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  • Water soluble extract of red sage root and its preeparation method and use
  • Water soluble extract of red sage root and its preeparation method and use
  • Water soluble extract of red sage root and its preeparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Salvianolic acid extract T of the present invention 1 preparation of

[0025] Take 1000 grams of Salvia miltiorrhiza, after pulverization, impregnate with 25% ethanol successively, each time for 24 hours, and repeat the impregnation twice (when impregnating, mechanically stir the impregnating solution for one hour). The impregnation liquids were combined and filtered, and the ethanol was distilled off under reduced pressure to obtain a concentrated aqueous solution, which was diluted with deionized water, centrifuged, and set aside. Get D-140 type non-polar macroporous resin packing column, above-mentioned aqueous solution is passed through this macroporous resin column with the flow velocity of 20 milliliters / minute, then wash column with the flow velocity of 20 milliliters / minute with 500 milliliters of deionized water, then use Eluted with 75% ethanol, combined the eluents, concentrated under reduced pressure, and dried in vacuum to obtain salvianolic acid...

Embodiment 2

[0026] Example 2 Salvianolic acid extract inhibits angiotensin-converting enzyme activity test

[0027] ① Preparation of angiotensin-converting enzyme

[0028] Rat lung tissue was taken, washed with 10 mmol / l ice potassium phosphate buffer, cut into small pieces, and homogenized with a homogenizer. The homogenate was centrifuged at 5000g for 10 minutes at 4°C, and the precipitate was discarded. The supernatant was dialyzed overnight with the above ice potassium phosphate buffer solution at low temperature, 2 liters each time, and the solution was changed 4 times. Then centrifuge at 40,000g for 40 minutes at 4°C, discard the precipitate, and the supernatant is the enzyme extract, which is aliquoted and stored at -20°C for future use.

[0029] ② Salvianolic acid extract T 1 Determination of the effect on angiotensin converting enzyme activity

[0030] Take 10ug / ml, 50ug / ml, 100ug / ml different concentrations of salvianolic acid extract T 1 10 μl and 10 μl of enzyme extract (...

Embodiment 3

[0035] Example 3 Salvianolic acid extract T 1 Effects on Blood Pressure of Renal Hypertensive Rats

[0036] Modeling method: male and female SD rats, weighing 200-220 grams, provided by the Experimental Animal Center. Rats were anesthetized by intraperitoneal injection of pentobarbital 30mg / kg, an incision was made on the left side of the rat waist, the left kidney was pulled out, the beginning of the renal artery was separated, and the renal artery was clamped with 0.2mm silver clips to narrow it and the renal artery was only separated but not narrowed with silver clips as a control (ie, sham-operated rat), and the skin was sutured. The blood pressure was measured once every 2 weeks after the operation, and the RBP-1B rat sphygmomanometer (developed by the China-Japan Friendship Hospital) was used to measure the systolic pressure of the tail artery. Each measurement was repeated three times, and the average value was taken as the blood pressure of the week. After 5 weeks, t...

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Abstract

A water-soluble extract of red sage root containing danshinolic acid B, danshensu and proshikonic acid, its preparing process, and its application in preparing the angiotonin converzyme depressant medicines for treating hypertension, and hypertension accompanied by heart failure or diabetes or nephrosis are disclosed.

Description

technical field [0001] The present invention relates to an extract of medicinal raw material and its preparation method and application. Specifically, it is a water-soluble phenolic acid extract obtained from the medicinal raw material Salvia miltiorrhiza, the extraction and preparation method of the extract, and an angiotensin-converting enzyme inhibitor drug using the extract as an effective medicinal ingredient, which can be used as a therapeutic Drugs for high blood pressure, heart failure, diabetic kidney disease and other diseases. Background technique [0002] Salvia miltiorrhiza is the dried rhizome of Salvia miltiorrhiza, a plant of the Labiatae family, and is a commonly used traditional Chinese medicine in clinical practice. The chemical composition of Salvia miltiorrhiza is mainly composed of fat-soluble components and water-soluble components. Among them, the fat-soluble components are represented by tanshinone. Pharmacological and clin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P3/10A61P9/12A61P13/12C07C51/47C07C59/52C07D307/80
Inventor 高小平徐大勇刘忠荣李伯刚
Owner CHENGDU DIAO PHARMA GROUP
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