Production method of high purity oligoxylose

A technology of xylo-oligosaccharide and production method, applied in the direction of oligosaccharide and the like, can solve the problems of high equipment requirements and serious environmental pollution, and achieve the effects of high product value, obvious industrialization and mild production conditions

Active Publication Date: 2004-12-22
河南益常青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, 4-20% strong alkali was used to extract xylan, which requires high equipment and serious environmental pollution, so it is not suitable for industrialization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the production of high-purity xylo-oligosaccharide syrup

[0020] Corncobs are crushed and soaked in 0.5% (W / W) sulfuric acid at 50°C for 12 hours, then the liquid is filtered out, the solid phase is cooked at 150°C for 1 hour, and then mixed with 1:10 (W / W) Proportionally add water for beating, adjust the pH of the obtained mixed phase to 6.5, add xylanase, the dosage of xylanase is 20 IU / g corn cob, and hydrolyze at 50° C. for 24 hours. After the reaction is finished, filter out the corncob residue with silk cloth to obtain the filtrate, which is the enzymatic hydrolysis solution. The enzymolyzed solution passes through a nanofiltration membrane with a relative molecular mass cut-off of 150 after being refined, and then passes through an ultrafiltration membrane with a relative molecular mass cut-off of 10,000, and then is refined and finally concentrated to 70 ° Bx to obtain a xylooligosaccharide content of 94.1% of the syrup product was spray-dried to...

Embodiment 2

[0023] Embodiment 2: the production of high-purity xylo-oligosaccharide syrup

[0024] Corncobs were crushed and soaked in 0.3% (W / W) sulfuric acid at 80°C for 8 hours, then the liquid was filtered off, the solid phase was cooked at 130°C for 4 hours, and then mixed with 1:10 (W / W) Proportionally add water for beating, adjust the pH of the obtained mixed phase to 6.5, add xylanase, the dosage of xylanase is 30IU / g corncob, and hydrolyze at 50°C for 12 hours. After the reaction is finished, filter out the corncob residue with silk cloth to obtain the filtrate, which is the enzymatic hydrolysis solution. After refining, the enzymatic solution passes through an ultrafiltration membrane with a relative molecular mass cut-off of 10,000, and then through a nanofiltration membrane with a relative molecular mass cut-off of 150, and then refines and concentrates to obtain a syrup product with a xylooligosaccharide content of 93.7%. Spray-dried to obtain a powder product.

[0025] ...

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PUM

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Abstract

A high-purity oligoxylose is prepared from corn cob through pretreating by diluted acid, digestion, enzymolyzing by xylanase, refining, ultrafiltering, and nano filtering. Its advantages are high purity (90%) and low cost.

Description

technical field [0001] The invention relates to a production method of high-purity xylo-oligosaccharides, in particular to using corncobs as raw materials, enzymatically hydrolyzing the xylan in the cooked corncobs, and then using ultrafiltration and nanofiltration techniques to obtain high-purity xylan Xylo-oligosaccharide products belong to the application field of enzyme engineering and membrane separation technology in the food industry. Background technique [0002] Xylooligosaccharide, also known as xylooligosaccharide, is a food functional factor that can proliferate bifidobacteria and improve the environment of intestinal flora, and is widely used in the production of food and feed. Xylooligosaccharide is a mixture of oligosaccharides composed of 2 to 7 xylose molecules connected by β-1,4-glycosidic bonds, of which xylobiose and xylotriose are the main active ingredients. [0003] The sweetness of xylooligosaccharide is about 50% of that of sucrose, and the sweetnes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C08B37/00
Inventor 杨瑞金许时婴王璋张闯黄海
Owner 河南益常青生物科技有限公司
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