Co-loaded gene and medicine super micro carrier particles and its preparing method
A technology of carrier particles and ultra-fine particles, applied in the direction of powder transportation, etc., can solve the problems that have not yet been seen, and achieve the effects of improving drug efficacy, uniform particle size distribution, and improving quality of life.
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Embodiment 1
[0045] The preparation method of ultrafine particles carrying drug surface adsorption genes inside is adopted, and the drug in this example is 5-Fu. 0.25g5-Fu was dissolved in 500 μl deionized water to obtain solution A, B was the dichloromethane solution of 1ml2% PLGA, C was 5ml0.3% O-carboxymethyl chitosan solution, and E was 20 μg 100 μl plasmid DNA ( antisense epidermal growth factor receptor). The 5-Fu solution was added into the PLGA solution under the condition of ultrasonic dispersion (200W, 30S). The O-carboxymethyl chitosan solution was added dropwise into the colostrum solution under the condition of ultrasonic dispersion (60W, 20S). The solvent of the double emulsion was evaporated for 3 hours under the condition of stirring (1,200r / min). The obtained particles were collected by centrifugation at 12,000 r / min for 15 minutes in a high-speed centrifuge, washed 3 times with double distilled water, and then freeze-dried for 24 hours for use. Accurately weigh 600 μg ...
Embodiment 2
[0048] The difference from Example 1 is that B is the acetone solution of 1ml 6% PLA. The 5-Fu solution was added to the PLA solution under stirring (800r / min). The O-carboxymethyl chitosan solution was added dropwise into the colostrum solution under stirring condition (1000r / min). The solvent of the double emulsion was evaporated for 3 hours under the condition of stirring (1,200r / min). The obtained particles were collected by centrifugation at 12,000 r / min for 15 minutes in a high-speed centrifuge, washed 3 times with double distilled water, and then freeze-dried for 24 hours for use. Accurately weigh 600 μg of the lyophilized particles and disperse them in double distilled water, and then place them in a constant temperature water bath at 55° C. for 10 minutes. The plasmid DNA (antisense EGFR) was then placed in a constant temperature water bath at 55° C. for 10 minutes. The two are quickly mixed, mixed with a shaker for 10 seconds, and then placed in a constant tempera...
Embodiment 3
[0050] The preparation method of ultrafine particles that simultaneously encapsulate drugs and genes is adopted, and the drugs in this example are 5-Fu and plasmid DNA (antisense epidermal growth factor receptor). A is 0.25g 5-Fu 500μl solution and 25μg 100μl plasmid DNA solution, B is 1ml 2% PLGA in dichloromethane solution, C is 5ml 0.3% O-carboxymethyl chitosan solution. E is 20 μg in 100 μl plasmid DNA. The 5-Fu solution and the plasmid DNA solution were added to the PLGA solution under the condition of ultrasonic dispersion (40W, 20S). The O-carboxymethyl chitosan solution was added dropwise into the colostrum solution under ultrasonic dispersion (60W, 20S). The solvent was volatilized for 3 hours while the complex emulsion was loaded with stirring (1,200r / min). The obtained particles were collected by centrifugation at 12,000r / min in a high-speed centrifuge for 15 minutes, washed 3 times with double distilled water, and then freeze-dried for 24 hours to collect the par...
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