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Method for extracting Gexianmi amylose from nostoc

An extraction method and a culture solution technology, which are applied in the fields of biotechnology and applied algae, can solve the problems of high cultivation cost, human harm, environmental pollution, etc., and achieve the effects of simple equipment, good product quality and high output.

Inactive Publication Date: 2005-01-12
INST OF AQUATIC LIFE ACAD SINICA
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AI Technical Summary

Problems solved by technology

Most of the currently used algae polysaccharides are extracted from macroalgae and spirulina, and the cultivation cost of these algae is high.
[0003] Most of the existing methods of extracting algal polysaccharides use chloroform and n-butanol to extract, and chloroform is harmful to the human body. If chloroform is not fully utilized during the extraction process, it will be directly discharged into rivers and lakes, which will pollute the environment

Method used

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Examples

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Embodiment Construction

[0023] The specific implementation steps of extracting kudzu rice polysaccharide are as follows:

[0024] A. Preparation of culture medium:

[0025] Add 1.5g of sodium nitrate, 0.04g of dipotassium hydrogen phosphate, 0.075g of magnesium sulfate, 0.036g of calcium chloride, 0.006g of citric acid, 0.006g of ferric ammonium citrate, and disodium salt of ethylenediaminetetraacetic acid (EDTA) per liter of water 0.001g, sodium carbonate 0.02g, trace element solution A51ml;

[0026] B, the cultivation of Ge Xianmi:

[0027] The cultivation conditions of Gexianmi are a very important determining condition for the yield of polysaccharides. Therefore, the whole growth process of Gexianmi is best cultivated according to the following conditions.

[0028] 1. First-level culture: transfer the original seed of the test tube to the Erlenmeyer flask (150-200ml) for static culture or aerated culture. If aerated culture is used, adjust the airflow to 3L.min -1 , the airflow passes through...

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Abstract

The invention discloses a method for extracting nostoc polysaccharide. Said method includes the following steps: firstly, adopting dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, citric acid, ammonium ferric citrate, disodium EDTA, sodium carbonate and minor element solution A5 to prepare culture liquor of nostoc according to a certain ratio; then controlling temp. light intensity and deration condition of the culture liquor to make culture of nostoc; third ste, harvesting nostoc; fourth ste, extracting nostoc polysaccharide; and fifth step, purifying, harvesing and drying nostoc polysaccharide. Said invention method is easy to implement, its operation is convenient.

Description

technical field [0001] The invention relates to the fields of biotechnology and applied phycology, and more specifically relates to a method for extracting polysaccharides from kudzu rice. Background technique [0002] At present, the development of kudzu rice only stays in the use of cultivation as food, and it is almost never involved in extracting polysaccharides from kudzu rice and utilizing them. Its polysaccharide has the ability to form a stable gel; it can stabilize suspensions and emulsions, increase the viscosity of the solution, form fibers, glue layers, and liquid crystals; it has the effect of biological flocculants. Therefore they can be used in food industry, pharmaceutical industry, cosmetic industry, textile industry and petroleum industry etc. Most of the currently used algae polysaccharides are extracted from macroalgae and spirulina, and the cultivation cost of these algae is high. [0003] Most of the existing methods for ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘永定黄泽波谢作明沈银武李敦海陈兰洲
Owner INST OF AQUATIC LIFE ACAD SINICA
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