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Yuzu Sea-cucumber osamine glycan injecta and its preparation method

A technology of yuzu sea cucumber and glycosaminoglycan, which is applied in the field of preparation of the injection, can solve the problems of different molecular weights, inability to use first aid for blood embolism diseases, poor safety, etc.

Inactive Publication Date: 2005-02-16
黑龙江红豆衫药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to treat or prevent such diseases, the pharmaceutical industry has developed some drugs. Although the current clinical drugs for the treatment of blood embolism have different curative effects, they also have the disadvantages of single indication, large side effects and poor safety.
The glycosaminoglycan of sea cucumber yuzu contained in sea cucumber yuzu has the function of treating blood embolism diseases, but there are still the following problems in the use of glycosaminoglycan of sea cucumber yuzu in pharmaceuticals: 1. glycosaminoglycan of sea cucumber yuzu is a kind of A substance with a wide molecular weight distribution. Different molecular weights have different efficacy in treating blood embolism diseases.
What molecular weight range of sea cucumber glycosaminoglycan is most suitable for pharmacy has not been reported so far; Oral agents rather than injections for prevention or long-term treatment, the disadvantage is that the curative effect is not satisfactory, especially it cannot be used for emergency treatment of blood embolism diseases
Conventional trypsin enzymatic extraction of glycosaminoglycans from sea cucumber yuzu has the disadvantages of low product purity, low yield and long process route
In addition, this conventional process method is also difficult to control the molecular weight of the product obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 10kg of dried sea cucumber yuzu, fully soak it, and grind it into fragments of ≤3mm, add 70kg of water and hydrolyze 200ml of protease for enzymolysis, and centrifuge the enzymolysis product at a temperature of 4°C (rotating speed: 4000 rpm , 20 minutes), extract the supernatant after separation. After removing the acidic protein in the supernatant under the condition of pH 2.5 (adjust the pH with hydrochloric acid, let it stand for 2 hours to remove precipitation), and then centrifuge it at a temperature of 4°C (rotating speed 4000 rpm, 20 minutes), the supernatant was taken. The supernatant was alcohol-precipitated (pH 7.0) with ethanol to extract the precipitate. Dissolve the precipitate with distilled water, and make it light yellow with hydrogen peroxide (3% V / V) at pH 11. After the decolorized solution was centrifuged at a temperature of 4°C (rotating speed 4000 rpm, 20 minutes), the supernatant was put forward, and the supernatant was precipitated with eth...

Embodiment 2

[0034] The glycosaminoglycan of yuzu sea cucumber freeze-dried in Example 1 was dissolved in water for injection to obtain an aqueous injection, and the weight ratio of glycosaminoglycan of yuzu sea cucumber to water for injection was 90:10.

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PUM

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Abstract

The invention provides an injection solution for curing blood embolism diseases, it contains sea cucumber glycosaminoglycan. Above 93% of the sea cucumber glycosaminoglycan is the one with molecular weight of 100000-140000. The injection solution can be freeze-dry injection solution and water injection solution. The invention also provides the manufacturing method for above mentioned injection solution, it contains steps of crash, immerse, enzyme decomposing and depositing of sea cucumber.

Description

Technical field: [0001] The invention relates to a glycosaminoglycan injection of yuzu sea cucumber and a preparation method of the injection. technical background: [0002] Blood embolism diseases, such as ischemic cardiovascular and cerebrovascular diseases, pulmonary thrombosis, venous thrombosis, etc., are frequent and common diseases that threaten human health. In order to treat or prevent such diseases, some medicines have been developed in the pharmaceutical industry. Although the medicines currently used in clinical treatment of blood embolism have different curative effects, they also have disadvantages such as single indication, large side effects and poor safety. The glycosaminoglycan of sea cucumber yuzu contained in sea cucumber yuzu has the function of treating blood embolism diseases, but there are still the following problems in the use of glycosaminoglycan of sea cucumber yuzu in pharmaceuticals: 1. glycosaminoglycan of sea cucumber yuzu is a kind of A subs...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K9/19A61K31/726A61P7/02
Inventor 陈任重汪寄宇
Owner 黑龙江红豆衫药业有限责任公司