Stabilization technology for intact phycobilisomes of red algae and blue algae

A technology of phycobilisomes and red algae, which is applied in the field of marine biology, can solve the problems of easy dissociation, difficulty in long-term storage of phycobilisomes, and restrictions on the application of phycobilisomes, achieving good application prospects
CN1654627AInactive Publication Date: 2005-08-17SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2005-08-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The intact cyanophyta and red algae phycobilisome stabilizing technology belongs to the field of marine biotechnology. The cyanophyta and red algae material has the cells ultrasonic wave crushed, the phycobilisome dissociated from the thylakid membrane with detergent TritonX-100, and detergent TritonX-100, chlorophyll and cell fragment eliminated through conventional centrifugation. The intact cyanophyta or red algae phycobilisome is prepared through ultracentrifugal sucrose density gradient process, and protected integrally via non-covalent bond coating process with polysaccharide and gluicosan or covalent bond formaldehyde crosslinking process. The protected phycobilisome may be preserved at room temperature for 30 integrally without dissociation, and the present invention lays foundation for applying phycobilisome in super-sensitive biomedicine.
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Description

(1) Technical field

[0001] The invention relates to the stabilization technology of complete phycobilisomes of cyanobacteria and red algae, and belongs to the technical field of marine biology. (2) Background technology

[0002] Phycobiliprotein is a kind of photosynthetic light-harvesting pigment-protein complex, which mainly exists in cyanobacteria, red algae, cryptophyta and a few dinoflagellates. It plays a role in capturing and transmitting light energy in photosynthesis, and has strong fluorescence. In cyanobacteria and red algae, the supramolecular structure phycobilisome is composed of 2-3 kinds of phycobiliproteins, forming an efficient energy transfer sequence. In the mid-1980s, American scholars who studied algae photosynthesis proposed to use phycobiliprotein as a fluorescent marker for diagnostic reagents. Compared with commonly used fluorescent markers, phycobiliprotein has the following advantages: the production process is safe, non-toxic, strong light ener...

Claims

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