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Applications of tumor-placenta antigen protein and its DNA in tumor treatment

A technology of tumor antigen and DNA sequence, applied in the direction of anti-tumor drugs, antibody medical components, peptide/protein components, etc., can solve the problems of difficult control of tumors, poor treatment effect and prognosis, and difficult to cure, so as to prevent and treat tumor cell metastasis Effect

Inactive Publication Date: 2005-09-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These tumors grow rapidly and are difficult to cure even if they are found. At present, the main treatment methods are local treatments such as traditional surgical resection and radiotherapy. However, most patients are difficult to control when they are diagnosed, and they are quite resistant to radiotherapy and chemotherapy.
Therefore, the effect and prognosis of treatment are often poor, and the one-year and five-year survival rates of patients are very low.

Method used

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  • Applications of tumor-placenta antigen protein and its DNA in tumor treatment
  • Applications of tumor-placenta antigen protein and its DNA in tumor treatment
  • Applications of tumor-placenta antigen protein and its DNA in tumor treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Discovery of Differentially Expressed cDNA Fragment PLAC1 in Liver Cancer

[0039] In order to screen differentially expressed genes in primary hepatocellular carcinoma, we used suppression subtractive hybridization (suppression subtractive hybridization, SSH) kit (PCR-SelectTM cDNA Subtraction Kit) from Clontech Company, and used suppression subtractive hybridization technology to obtain liver cancer tissue As a tester (tester), the corresponding paracancerous tissue as a driver (driver), carried out suppression subtractive hybridization.

[0040] Firstly, the total RNA of liver cancer and adjacent tissues was extracted with TRIzol kit (Gibco Company), mRNA was isolated with PolyATract mRNA isolation kit of Promega Company (Promega, Madison, WI), and the concentration and purity of mRNA were detected by ultraviolet spectrophotometry.

[0041] The SSH operation process is according to the kit instructions. Synthesize the first and second strands of cDNA with ...

Embodiment 2

[0043] Embodiment 2, using the method of RT-PCR to prove the expression of PLAC1 gene in liver cancer, intestinal cancer, gastric cancer and lung cancer

[0044] cDNA of 16 commercial human normal tissues from the biotechnology company CLONTECH (spleen, prostate, testis, ovary, small intestine, large intestine, white blood cells, heart, lung, liver, brain, kidney, pancreas, placenta, skeletal muscle, thymus), cDNAs from cancer tissues and matched paracancerous tissues of 39 liver cancer patients, cDNAs from cancer tissues and matched paracancerous tissues of 24 intestinal cancer patients, cancer tissues and matched cancer tissues of 24 gastric cancer patients The cDNA of paracancerous tissue, the cDNA of cancer tissue and paired paracancerous tissue from 24 cases of lung cancer patients were used to detect the expression of PLAC1 gene in normal tissue and tumor tissue.

[0045] The conditions for RT-PCR in the present invention: use the advanced hot-start DNA polymerase of CLO...

Embodiment 3

[0046] Example 3. Using Northern hybridization to analyze the expression of CP1 gene in tumor tissues of liver cancer, intestinal cancer, gastric cancer and lung cancer

[0047] Total RNA was extracted from cancerous tissues and paired paracancerous tissues of tumor patients with TRIZOL reagent (PROMEGA), and then mRNA was isolated from total RNA using mRNA isolation kit (PROMEGA). In the presence of formamide and formaldehyde, 2ug of mRNA was denatured, after agarose electrophoresis, it was transferred to Hybond-N+ nylon membrane (Amersham), and fixed by ultraviolet crosslinking. The DNA fragments amplified in Example 2 were labeled with Digoxigenin DNA Probe Labeling Kit (Roche) to make DNA probes, hybridized with the mRNA on the membrane according to the conventional Northern hybridization method, and then carried out autoradiography, The mRNA of the tumor-placenta antigen protein gene CP1 in the present invention was detected. Then, boil the membrane used to detect the mR...

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Abstract

The invention relates to the use of tumor-placenta antigen protein in preparing medicament for treating cancer, the invention also relates to the use of the DNA sequence encoding the tumor-placenta antigen protein in preparing medicament for treating cancer, the tumor antigen protein can provide a new way for tumor treatment and the diagnosis of tumor occurrence, development and transfer.

Description

technical field [0001] The present invention relates to a tumor antigen protein and the application of its coding DNA, in particular to the application of a tumor-placenta antigen protein and its DNA in the preparation of medicines for treating liver cancer, intestinal cancer, gastric cancer and lung cancer. technical background [0002] Malignant tumors are a large class of diseases that threaten human health. Tumor occurrence is a very complex process. An important factor for the occurrence and progression of tumor cells is that they can evade the surveillance of the body's immune system. Therefore, how to stimulate and evoke the body's immune system to recognize and reject tumor cells with "non-self" components is the key to finding a new tumor treatment method A breakthrough. In the initial stage of immunotherapy research on tumors, people have developed many methods to stimulate the body's immune system to reject tumor cells with "non-self" components. Although these ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61K39/00A61K45/00A61P35/00
Inventor 陈慰峰董学员庞学雯
Owner PEKING UNIV
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