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Use of human lysozyme in preparing medicines for cutaneous pruritus

A technology of human lysozyme and pruritus, which is applied in the field of application of human lysozyme in the preparation of medicines for treating pruritus, and can solve problems such as skin burning, tingling, large side effects, and skin damage

Active Publication Date: 2005-10-12
张华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 2. External factors
At present, the commonly used therapeutic drugs for pruritus, such as "Lefu Ye" produced by Tianjin Tianjiao Pharmaceutical Co., Ltd., have very serious side effects, such as burning and tingling sensations when rubbed on the skin, and those with skin damage are more serious. Long-term use has hormonal effects and affects health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Recombinant human lysozyme is based on the preparation of 200 ml medium, with 6 ml phosphoric acid, 3 g magnesium sulfate, 4 g potassium sulfate, 1 g potassium hydroxide, 1.5 g calcium sulfate, add distilled water to 200 ml, after autoclaving Glycerol tube seeds were inoculated, the rotation speed of the shaker was 250 revolutions per minute, the cultivation temperature was 20° C., and the cultivation was carried out on a constant temperature bed for 36 hours. The seed tank culture is carried out, and finally the production tank culture is carried out. Extracting and purifying the culture medium after fermentation and expression, freeze-drying the extracted and purified gene recombined human lysozyme concentrate, measuring protein quantity, purity and preservation of lysozyme activity.

[0063] The recombinant human lysozyme with a purity of 95% was prepared at 1500U / mL / g, phosphate buffer 10-20mM (pH6.5-7.5) 80%, 15% propylene glycol, 1% water-soluble azone, 5 / 10000 T...

Embodiment 2

[0065] According to the method described in Example 1, the recombinant human lysozyme was obtained, and the recombinant human lysozyme with a purity of 97% was obtained at 30000 U / mL / g, and the phosphate buffer was 10-20 mM (pH6.5-7.5) 84%, 13% propylene glycol, 2% water-soluble azone, and 5 / 10,000 Tween 80 are mixed homogeneously at room temperature, and are made into drops according to pharmaceutical regulations in a pharmaceutical factory meeting GMP requirements.

Embodiment 3

[0067] According to the method described in Example 1, the recombinant human lysozyme was obtained, and the recombinant human lysozyme with a purity of 98% was obtained at 100,000 U / mL / g, and the phosphate buffer was 10-20 mM (pH6.5-7.5) 78%, 21% propylene glycol, 1% water-soluble azone, and 5 / 10,000 Tween 80 are mixed homogeneously at room temperature, and are made into drops according to pharmaceutical regulations in a pharmaceutical factory meeting GMP requirements.

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Abstract

An application of human lysozyme in preparing the medicine in the form of spray, drops, or ointment for treating itching is disclosed. Its advantages are no irritation and anaphylactic reaction and high curative effect and safety.

Description

Technical field: [0001] The invention relates to a new application of human lysozyme, in particular to a new application of human lysozyme in the field of medicine. Background technique: [0002] Pruritus is seen in a variety of inflammatory or non-inflammatory skin diseases, and the pathogenic factors are relatively complex, which can be divided into pruritus caused by internal and external factors. [0003] 1. Internal factors [0004] (1) Senile skin changes: the skin ages accordingly with age, sweat glands and skin glands atrophy, secretion function decreases, the hydration capacity of the stratum corneum decreases, the water-fat emulsion on the skin surface decreases, and the skin becomes dry due to lack of moisture. [0005] (2) Liver disease: cholestatic pruritus. [0006] (3) Kidney disease: Most patients with chronic renal failure have generalized itching in varying degrees. [0007] (4) Endocrine and metabolic diseases: Patients with hyperthyroidism may be accom...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K38/47A61P17/04
Inventor 张华
Owner 张华
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