Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for refining sheep placenta active component using membrane separating technology

A technology of active ingredients and refining methods, which is applied in the field of membrane separation technology to extract and separate sheep fetal active substances, to achieve the effects of energy saving, high extraction yield and high efficiency

Active Publication Date: 2005-10-19
JIANGZHONG PHARMA
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in terms of operation technology and extraction purity, the above methods all have defects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Mince the fresh or frozen lamb embryos and placenta that have passed the quarantine inspection into meat sauce, beat at low temperature, weigh 2000 grams of the above-mentioned extracted slurry and put it into the enzymatic hydrolysis tank, start the stirrer to stir, heat up to 50°C, weigh 3 grams of neutral protease, hydrolyzed for 40 minutes, inactivated at 75°C for 5 minutes, and immediately microfiltered the above extract with a zirconia ceramic membrane with a cut-off of 0.1um at a filtration pressure of 0.1Mpa, and collected the permeated The liquid is then concentrated by nanofiltration. The nanofilter adopts a polyethersulfone composite membrane with a molecular weight cut-off range of 150-180 and a filtration pressure of 0.1-0.2Mpa. Most of the water is removed to obtain 500 ml of sheep fetus concentrate, which is then freeze-dried , the yield of sheep fetus extract was 6.5%, and the measured peptide content was 33.8%.

Embodiment 2

[0023] Sheep embryo and placenta pretreatment, low-temperature homogenization, and enzymatic hydrolysis process are the same as in Example 1. Immediately centrifuge and filter at a speed of 4000-5000 rpm for 20 minutes. The clear liquid is subjected to microfiltration with a filtration pressure of 0.1Mpa. The permeate is collected and then concentrated by nanofiltration. The nanofilter uses a polyethersulfone composite membrane with a molecular weight cut-off range of 150-180 and a filtration pressure of 0.1-0.2Mpa. Most of the water was removed to obtain 520 ml of sheep fetal concentrate, and then freeze-dried. The yield of sheep fetal extract was 6.1%, and the measured peptide content was 32.4%.

Embodiment 3

[0025] Sheep embryo and placenta pretreatment, homogenization, and enzymatic hydrolysis process are the same as in Example 1, and they are centrifuged and filtered immediately at a speed of 4000-5000 rpm for 20 minutes. The above-mentioned centrifuged supernatant is separated by a polysulfone membrane with a cut-off of 0.08um. The liquid is subjected to microfiltration, the filtration pressure is 0.1Mpa, the permeate is collected and then concentrated by nanofiltration. 700 milliliters of sheep fetus concentrate was obtained from part of the water, and freeze-dried. The yield of sheep fetus extract was 5.9%, and the measured peptide content was 30.8%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the extracting and separating method of active matters in sheep placenta, and is especially process of extracting active matters from sheep placenta with microfiltering film and nanofiltering film. The extracting process includes material collection, sorting, pulping, crushing, enzymolysis, film separation, and freeze drying. The film separating includes using inorganic ceramic film of aluminum oxide, zirconium oxide or antimony oxide or organic film of polysulfone, cellulose acetate, PTFE or polypropylene with intercepting particle range of 0.05-1 micron to eliminate hybrid protein, rib segment and residual degrading enzyme; and using nanofiltering film of polyamide, polypeperazine amide or polyether sulfone to intercept molecular weight 150-1000 components while eliminating free amino acid and water.

Description

technical field [0001] The invention is a method for extracting and separating the active substance of sheep fetus by adopting the membrane separation technology, especially a method for refining and extracting the active substance of sheep fetus by using a microfiltration membrane and a nanofiltration membrane. Background technique [0002] The placenta is an organ for material exchange between the fetus and the mother. It is a physiological product that supplies the fetus with complete nutrients. The protein content is more than 70%, the amino acid content is rich, the variety is complete, the proportion is reasonable, the fat content is about 15%, and it is rich in phospholipids. , lipopolysaccharide, and contains a variety of vitamins and essential constants and trace elements for the human body, as well as a variety of immunoglobulins, a variety of antibodies, as well as interferon closely related to immune function and active polypeptides re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50
Inventor 徐国良张俐伟粱艳琴宋琳田颖刚付玉梅邹胡斌
Owner JIANGZHONG PHARMA