Process for extracting folium mori flavone

An extraction method and technology of mulberry leaf flavonoids are applied in the extraction field of extracting flavonoid active components thereof, and the effects of large extraction amount, recyclable price and high extraction efficiency are achieved.

Inactive Publication Date: 2005-10-19
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the extraction method of extracting mulberry flavonoids from the medicinal plant mulberry leaves in large quantities. Based on the above reasons, the present invention has carried out detailed research on the extraction method of mulberry leaf flavonoids.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 500g of mulberry leaves, crush them through a 20-mesh sieve, and use 3 liters, 2 liters, and 2 liters of 80% ethanol solution to reflux three times for extraction, the first reflux for 2.0 hours, the second and third reflux for 1.0 hour respectively, combine the extracts, and refrigerate Stand still for 24 hours, filter, and concentrate the filtrate under reduced pressure to obtain the ethanol extract; extract the ethanol extract five times with water, 500ml each time, extract for 1 hour, keep the temperature at 75°C, combine the water extract, stand for filtration, and decompress Concentrate to about 2 liters; use hydrochloric acid to adjust the pH value to 3.5; add NaCl to make the salt content 3%; the concentrated solution is separated on a column with a column height of 60 cm, a column diameter of 3.0 cm, and a column packing of D 101 Macroporous adsorption resin, rinse with water until the effluent becomes clear, then rinse with 40% ethanol for 5 times the col...

Embodiment 2

[0023] Weigh 1Kg of mulberry leaves, crush them through a 40-mesh sieve, and use 5 liters, 4 liters, and 4 liters of 75% ethanol solution to reflux three times for extraction, the first reflux for 2.0 hours, the second and third reflux for 1.0 hour respectively, combine the extracts, and refrigerate Stand still for 24 hours, filter, and concentrate the filtrate under reduced pressure to obtain the ethanol extract; extract the ethanol extract five times with water, 800ml each time, extract for 1 hour, keep the temperature at 75°C, combine the water extracts, stand and filter; use hydrochloric acid Adjust the pH value to 4; add NaCl to make the salt content 3%; separate on the column, the column height is 60cm, the column diameter is 4.0cm, and the column packing is D 101 Macroporous adsorption resin; first rinse with water until the effluent becomes clear, then rinse with 50% ethanol for 4 times the column volume at a rate of 25ml / min, collect the eluent, concentrate under reduc...

Embodiment 3

[0025] Weigh 500g of mulberry leaves, crush them through a 20-mesh sieve, and use 3 liters, 2 liters, and 2 liters of 80% ethanol solution to reflux three times for extraction, the first reflux for 2.0 hours, the second and third reflux for 1.0 hour respectively, combine the extracts, and refrigerate Stand still for 24 hours, filter, and concentrate the filtrate under reduced pressure to obtain the ethanol extract; extract the ethanol extract five times with water, 500ml each time, extract for 1 hour, keep the temperature at 75°C, combine the water extract, stand for filtration, and decompress Concentrate to about 2 liters, use hydrochloric acid to adjust the pH value to 3.5; add NaCl to make the salt content 2%; the concentrated solution is separated on a column with a column height of 60 cm and a column diameter of 3.0 cm, and the column packing is AB-8 macroporous adsorption Resin, first rinse with water until the effluent becomes clear, then use 40% ethanol to rinse for 5 t...

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Abstract

The mulberry leaf flavone extracting process adopts mulberry leaf powder material, alcohol and water as extracting solvent and eluant and macroporous resin as separating material. The extract has mulberry leaf flavone content over 50 %. The present invention has the advantages of non-toxic cheap recoverable solvent, no environmental pollution, great extracted amount, capacity of industrial production and high extracting efficiency.

Description

Technical field: [0001] The invention relates to an extraction method for extracting flavonoid active ingredients from natural medicinal plant mulberry leaves. Background technique: [0002] Mulberry leaf, also known as iron fan, is the leaf of Moraceae mulberry (Morus alba L.). Mulberry leaves are sweet, bitter and cold, and return to the lung and liver meridian. Modern pharmacology has confirmed that mulberry leaves can inhibit the rise of blood sugar and have the effect of preventing and treating diabetes. Mulberry leaves are rich in flavonoids such as rutin, quercetin, isoquercetin and quercetin-3-glucoside, with a content of about 3%. Pharmacological studies have found that mulberry leaf flavonoids have strong anti-oxidation function, hypolipidemic function and significant hypoglycemic effect by inhibiting the activity of small intestinal disaccharidase in rats. In view of the diverse biological activities of mulberry leaf flavonoids, mulberry leaf flavones have broa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P3/06A61P3/10A61P39/06C07D311/30C07H1/08C07H17/07
Inventor 张立伟杨林
Owner SHANXI UNIV
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